Effects of salvianolic acid B on the energy metabolism and hydrocephalus in mice with cerebral ischemia |
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| Authors: | JIANG Yu-feng WANG Qiu-hua LIU Zhi-qin CAO Xu -quan REN Li-wei CAI Da-yong LIU Shi-jing HUANG Qi-fu |
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| Institution: | 1.Department of Pathology,Beijing University of Chinese Medicine,Beijing 100029,China;2.Department of Pharmacology,Pharmacological College of Hebei University,Baoding 071002,China.E-mail: jiangyf66@yahoo.com.cn |
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| Abstract: | AIM: To explore the effects of salvianolic acid B (SalB) on the energy metabolism and hydrocephalus in mice with cerebral ischemia.METHODS: NIH mice were randomly divided into four groups: sham-operated group,cerebral ischemia group,SalB-treated group and nimodipine-treated group.The brain tissue energy charge (EC),phosphocreatine (PCr),the activity of ATPase,excitability amino acid (EAA) content and water content of brain were measured when cerebral ischemia for 30 min.RESULTS: EC (0.520±0.034),PCr content ［(98.344±13.249) μmol/g］,the activity of Na+-K+-ATPase ［(0.593±0.013)×103 U/g］ and Ca2+-ATPase ［(0.484±0.053)×103 U/g］ in SalB-treated group were significantly higher than those in cerebral ischemia group {EC (0.465±0.037),PCr content ［(81.614±9.919) μmol/g］ ,the activity of Na+-K+-ATPase ［(0.244±0.065)×103 U/g］,the activity of Ca2+-ATPase ［(0.321±0.086)×103 U/g］} (P<0.01).The glutamate (Glu) content ［(0.405±0.110) μmol/g］,aspartate (Asp) content ［(0.141±0.020) μmol/g］ and water content of brain ［(38.1±0.1)%］ in SalB-treated group were markedly lower than those in cerebral ischemia group ［ Glu content (0.550±0.140) μmol/g,Asp content (0.287±0.050) μmol/g,water content of brain (44.1±0.1)%］ (P<0.05,P<0.01).CONCLUSION: The increase in cerebral energy metabolism and the activity of ATPase,and decrease in EAA content in brain tissue are the mechanism of SalB alleviating hydrocephalus at the early stage of cerebral ischemia in mice. |
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| Keywords: | Salvianolic acid B Brain ischemia Energy metabolism Excitatory amino acids Mice Brain edema |
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