Relationship Between Expression Levels of Porcine m6A Demethylases FTO and ALKBH5 Genes and Resistance to E.coli F18 Infection |
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| Authors: | QI Xiaoyi YANG Li WU Zhengchang BAO Wenbin WU Shenglong |
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| Institution: | 1. Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China;2. Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China |
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| Abstract: | To investigate the relationship between the mRNA expression level of m6A demethylase and E.coli F18 resistance in piglets,Real-time quantitative PCR was used to detect the mRNA expression differences of m6A demethylase FTO and ALKBH5 genes in the duodenum and jejunum tissues of E.coli F18-resistant and -sensitive individuals from 35-day Sutai weaned piglets.In E.coli F18ab,F18ac bacteria-stimulated and endotoxin LPS-induced porcine small intestinal epithelial cells (IPEC-J2),the expression levels of FTO and ALKBH5 genes were detected,respectively.The results showed that the expression levels of FTO and ALKBH5 genes in the duodenum and jejunum of resistant individuals were extremely significantly or significantly higher than those of sensitive individuals (P<0.01,P<0.05),and the expression of FTO gene were not significantly changed in E.coli F18 bacteria-stimulated IPEC-J2 cells (P>0.05),but the expression levels of ALKBH5 gene were significantly up-regulated after F18ac stimulation (P<0.05).After LPS induction for 4 hours,the expression levels of FTO and ALKBH5 genes showed significant up-regulation in IPEC-J2 cells (P<0.05).This study preliminarily verified and indicated that the expression levels of m6A demethylases FTO and ALKBH5 genes were closely related to E.coli resistance of piglets at the cellular and individual levels,which will provide a theoretical basis for future in-depth study of the regulation mechanism of RNA demethylation modification on bacterial diarrhea in piglets. |
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| Keywords: | pigs m6A demethylase FTO gene ALKBH5 gene E coli |
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