Effect of Overexpression of FOXL2 Gene on Gonadal Differentiation in Chicken Embryo |
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| Authors: | XU Wenwen XIA Li SUN Tiantian DENG Jixian YANG Zhuliang YANG Xiurong |
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| Institution: | 1. College of Animal Science and Technology, Guangxi University, Nanning 530004, China;2. Guangxi Institute of Animal Science, Nanning 530001, China |
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| Abstract: | The aim of this study was to investigate the effect of FOXL2 gene on gonadal differentiation in chicken embryos.This test was divided into the experiment groups 1,2 and blank control group(chicken embryos were 260,100 and 20,respectively).In the experimental group,pLV-FOXL2 lentivirus recombinant plasmids and pLV empty plasmids were injected into the chicken embryo on the second day of the embryonic stage by subpaneoidal injection.The blank control group was not treated and incubated with the experimental groups until the chick was hatched.CHD1 gene genetic sex identification method was used to detect the sex of chicks,the changes of gonadal anatomy and histological structure were analyzed,and the expression levels of FOXL2 and CYP19A1 proteins were detected by immunohistochemistry.The results showed that in the experiment group 1,there were 23 male and 18 female of genetic sex,21 male and 18 female of phenotypic sex,and two of them had atypical phenotypic gender,with changes in the left gonadal gland toward ovarian structure.The phenotypic sex was consistent with the genetic sex in experiment group 2,with 9 male and 12 female.Positive PCR results showed that 10 positive individuals were obtained in the experiment group 1,with a positive rate of 24.4% (10/41),and 8 positive individuals were obtained in the experiment group 2,with a positive rate of 38.1% (8/21).The anatomical structure of the gonad showed that the volume of the left gonad was significantly larger than that of the right gonad in the positive plV-FOXL2 male embryos.The results of tissue sections showed that the gonad of male chicken embryo had typical structure of ovarian cortex and medulla.There was no significant change in the development of gonad in female embryos with positive pLV-FOXL2.Immunohistochemical results showed that the expression levels of FOXL2 and CYP19A1 proteins in the left and right testicle of experiment group 1 were similar to that in the hen ovary of the blank control group,and were significantly higher than that in experiment group 2 (P<0.05).These results suggested that FOXL2 gene might promote the sexual inversion of chicken gonads and played an important role in the differentiation and development of chicken gonads. |
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| Keywords: | chicken embryo gonadal differentiation FOXL2 gene sex related genes |
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