Expression of IgG in bladder carcinoma and the effect of the antibody against human IgG on tumor cell apoptosis |
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| Authors: | LI Hao-yong LIANG Pei-yu ZHU Xiao-feng ZHENG Ke-li DAI Yu-ping |
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| Institution: | 1.Department of Urology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;2.Department of Urology, The Affiliated Hospital of Hainan Medical College, Haikou 570102, China;3.Research Department, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China. E-mail: haoyli5118@126.com |
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| Abstract: | AIM: To investigate the expression of immunoglobulin G (IgG) in bladder transitional carcinoma cells (BTCC) and the effect of the antibody against human IgG on tumor cell apoptosis.METHODS: The expression of IgG of BTCC was detected by immunohistochemistry, Western blotting, enzyme linked immunosorbent assay (ELISA) and flow cytometry (FCM). The expression of IgG mRNA was tested by hybridization in situ method and RT-PCR in vitro. Antiproliferation effects of the antibody of antihuman IgG on T24 and BIU-87 cell lines were measured by MTT assay. Cell apoptosis was assessed by FCM.RESULTS: The expression of IgG was 91.1％ (51/56) in clinical cases, and 45.4％ (5/11) in normal controls. Immunohistochemistry and Western blotting showed that the IgG was significantly expressed in T24 and BIU-87. FCM indicated the IgG was mainly expressed in cytoplasma. No IgG was detected by ELISA in supernatant of cell culture medium. RT-PCR and hybridization in situ demonstrated IgG mRNA was significantly expressed in two cell lines. Under the treatment of 25 mg/L of goat nonspecific IgG and the antibody of antihuman IgG, the inhibition ratio of cell growth in T24 and BIU-87 were (4.73±3.73)% vs (24.98±3.81)% and (5.36±1.53)% vs (22.70±3.72)%, respectively (P<0.05). The percentages of apoptotic cells in T24 and BIU-87 were 2.3% vs 20.7% and 1.3% vs 15.3%, respectively.CONCLUSION: IgG is significantly expressed in bladder transitional carcinoma cell. The antibody of antihuman IgG inhibits cancer cell growth and induces tumor cell apoptosis. |
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| Keywords: | Bladder neoplasms Immunoglobulin G Antibodies |
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