Preventive and Therapeutic Effects of Clostridium butyricum-longan Polysaccharide Fermentation Broth on Mouse Model with Ulcerative Colitis |
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| Authors: | XIANG Rong LIANG Weijie YUAN Minggui PENG Xinyu TIAN Ya WEI Qilin ZHOU Tingjin XU Zhihong |
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| Institution: | 1. Guangdong Provincial Key Laboratory of Animal Disease Prevention, Scientific Observing and Experimental Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;2. Zhaoqing Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Zhaoqing 526238, China |
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| Abstract: | The aim of the study was to observe the preventive and therapeutic effect of Clostridium butyricum-longan polysaccharide fermentation broth on mouse model with ulcerative colitis (UC).30 SPF male BALB/c mice were randomly divided into blank group,model group and fermentation broth group,10 mice/group.According to the dosage standard of 10 mL/kg body weight,sterile deionized water was administered to the blank group and model group,and Clostridium butyricum-longan polysaccharide fermentation liquid was administered to the fermentation broth group for 7 days.From the 8th day,the model group and the fermentation broth group were allowed to drink 5% dextran sulfate sodium (DSS) solution for 7 consecutive days to induce the UC model,and the blank group was free to drink sterile water.On the 14th day,all mice were weighed,and DAL scores and clinical scores were scored.Then the mice were dissected after eyeball collection and blood sampling.The length of the colon was measured and scored for histological damage of the colon,sections were made after formalin fixation.The content of IL-6,IL-10 and TNF-α in serum were detected by ELISA.The results showed that UC model was established successfully and symptoms were obvious in the model group.Compared with the model group,the weight loss of the mice in the fermentation broth group was significantly reduced (P<0.05),DAL score,clinical score,colon shortening degree and histological injury score were extremely significantly reduced (P<0.01).The colon tissue of mice in the fermentation broth group showed a relatively complete epithelial structure and crypts,and only part of the inflammatory cells invaded the epithelial tissue.ELISA results showed that the IL-6 and TNF-α levels in the model group were significantly higher than those in the fermentation broth group (P<0.05),and the IL-10 content was significantly lower than that in the fermentation broth group (P<0.05).It showed that Clostridium butyricum-longan polysaccharide fermentation broth had a certain preventive effect on DSS-induced UC in mice,and its mechanism might be related to reducing the content of IL-6 and TNF-α,increasing the content of IL-10,and inhibiting inflammation. |
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| Keywords: | Clostridium butyricum longan polysaccharide ulcerative colitis clinical symptoms |
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