Caspase 3 inhibitor Ac-DEVD-CHO attenuates 10-hydroxycamptothecin-induced activation of NF-κB in Bcap37 cells |
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| Authors: | YE Meng LIN Lei PAN Hong-ming FANG Yong WU Jin-min |
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| Institution: | 1.Department of Oncology,2Department of Dermatology,The Affiliated Hospital,Ningbo University,Ningbo 315020,China;3.Department of Oncology,Sir Run Run Shaw Hospital,Zhejiang University,Hangzhou 310016,China. E-mail: dryemeng@yahoo.com.cn |
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| Abstract: | AIM:To investigate the role of caspase 3 inhibitor Ac-DEVD-CHO in caspase 3 signaling pathway and NF-κB activation induced by 10-hydroxycamptothecin (HCPT) in human breast carcinoma cells. METHODS:The cell growth inhibition was measured by MTT assay. Agarose gel electrophoresis was performed for detecting cell apoptosis. Western blotting was used for determining protein expression. DIG-EMSA was conducted to measure the DNA-binding activation of NF-κB. RESULTS:Caspase 3 inhibitor Ac-DEVD-CHO attenuated HCPT-induced apoptosis in human breast carcinoma. Ac-DEVD-CHO also suppressed the degradation of caspase 3 and IκBα,and arrested the activation of NF-κB. CONCLUSION:Caspase 3 inhibitor Ac-DEVD-CHO regulates the activation of caspase 3 and NF-κB,and attenuates apoptosis in Bcap37 cell line induced by HCPT. |
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| Keywords: | Camptothecin Caspase 3 inhibitor Apoptosis NF-kappa B |
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