Porcine surfactant in treatment of LPS-induced early-stage acute lung injury in rats |
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| Authors: | WANG Yi-qun SUN Yu YANG Rui ZHONG Ji-gen LU Jian |
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| Institution: | Department of Pathophysiology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China |
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| Abstract: | AIM: To evaluate the therapeutic efficacy of intratracheal instillation of porcine pulmonary surfactant (PPS) in rats with lipopolysaccharides (LPS)-induced early-stage ALI in this study.METHODS: SD rats weighing 200 g-300 g were randomly divided into 4 groups: LPS (1.5 mg·kg-1)+saline,LPS+PPS 100 mg·kg-1,LPS+PPS 150 mg·kg-1,LPS+PPS 200 mg·kg-1.The PaO2 and PaCO2,as well as survival rate of rats were examined for 6 h after the start of PPS-instillation.Then,rats were killed and lungs were immediately removed for lung index (LI) and histological analysis.The bronchoalveolar lavage fluid (BALF) was collected for measurement of total protein (TP) contents,TNF-α level and white blood cell(WBC) numbers.RESULTS: Significantly increased PaO2,reduced mortality rate,decreased total protein and TNF-α contents in BAL,as well as lung index and meliorated histological appearance were observed in three PPS-treated groups compared with group given saline after LPS (P<0.05).The therapeutic effect in PPS150 and PPS200 groups was better than that in PPS100 group.CONCLUSION: Intratracheal PPS instillation provides protective effect on acute lung injury in rats induced by LPS. |
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| Keywords: | Acute lung injury Lipopolysaccharides Porcine pulmonary surfactants Rats |
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