Effect of CsA on the Expression of IL-1β and IL-6 in Endometrial Stomas Cells Induced by LPS |
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| Authors: | CHEN Mutian REN Yan ZHANG Lei LI Jing XIE Sijie ZHANG Yan ZHANG Ming MEI Xiuli |
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| Institution: | 1. College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang 611130, China;2. Sichuan Animal Science Academy, Chengdu 610066, China |
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| Abstract: | The aim of this study was to investigate the effect of immunosuppressant cyclosporine A (CsA) on the expression of proinflammatory cytokines interleukin 1 beta (IL-1β) and interleukin 6 (IL-6) in LPS-induced uterine endometrial stromas cells (ESCs).After cultured and identified in vitro,the inflammation model of the ESCs of female rabbits were successfully constructed by 1 μg/mL lipopolysaccharide (LPS),and 0 μg/mL LPS was used as the control.Then the expression levels of IL-1β and IL-6 were detected after treatment with 0,50,100,200 and 500 ng/mL CsA.The results showed that LPS significantly induced the expression of IL-1β and IL-6 in ESCs (P<0.05).Although CsA had no effect on the expression of IL-1β and IL-6,but 50,100,200 and 500 ng/mL CsA extremely significantly inhibited the upregulation of IL-1β and IL-6 induced by 1 μg/mL LPS (P<0.01).It suggested that a certain dose of CsA could effectively inhibit the LPS-induced immune stress in ESCs.This study provided a basis for the feasibility of CsA treatment after the genital tract of female infected by gram-negative bacteria,but its clinical application and mechanism still need to be further studied. |
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| Keywords: | cyclosporine A lipopolysaccharide interleukin 1 beta interleukin 6 immune stress |
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