Neuropeptide Y induced redistribution of intracellular free calcium in rat cardiomyocytes |
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Authors: | XIONG Long-gen ZHANG Yi SU Tao LIU Shi-ming DONG Qi CHEN Min-sheng |
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Institution: | 1.Department of Cardiology, 2 Institute of Neuroscience, The Second Affiliated Hospital, Guangzhou Medical College, Guangzhou 510260, China;3.Depatrment of Physiology, Guangzhou Medical College, Guangzhou 510182, China. E-mail: gzminsheng@vip.163.com |
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Abstract: | AIM: To investigate the effect of neuropeptide Y (NPY) on intracellular free calcium([Ca2+]i) and Ca2+ sarcoplasmic reticulum of cardiomyocytes in rats.METHODS: Cardiomyocytes of neonatal Sprague-Dawley rats were incubated with NPY at concentration of 100 nmol/L for 24 h. Fluorescent indicator Fluo-4 AM was used to detect [Ca2+]i and Fluo-5N AM was used to detect Ca2+ in sarcoplasmic reticulum (SR). Calcium image was recorded by laser scanning confocal microscope. The SR Ca2+ load was estimated by caffeine-induced Ca2+ transient (CCT). RESULTS: 24 h after incubation with NPY, compared with control group, the concentration of [Ca2+]i was significantly elevated (P<0.05), and the concentration of free Ca2+ in SR ([Ca2+]SR) was significantly decreased (P<0.05), and the peak of CCT was attenuated.CONCLUSION: Stimulation with NPY for 24 h causes redistribution of free calcium in rat cardiomyocytes, namely the elevation in [Ca2+]i and decline in [Ca2+]SR. |
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Keywords: | Neuropeptide Y Cardiomyocytes Sarcoplasmic reticulum Calcium |
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