| 海藻糖在双歧杆菌冻干菌粉制备和保藏中生物保护作用的初探 |
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| 引用本文： | 田洪涛,张篪,贾英民.海藻糖在双歧杆菌冻干菌粉制备和保藏中生物保护作用的初探[J].河北农业大学学报,2000,23(2):62-65. |
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| 作者姓名： | 田洪涛 张篪 贾英民 |
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| 作者单位： | [1]河北农业大学食品科学系,河北保宁 [2]中国农业大学仪器科学与工程学院,北京 |
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| 摘 要： | 对新型保护剂海藻糖在长双歧杆菌（Ｂｉｆｉｄｏｂａｃｔｒｅｒｉｕｍ ｌｎｇｕｍ）冻干菌粉制备和保藏中的生物保护作用进行了初步研究。结果表明：４种双糖保护剂中，涨藻糖的细胞冻干存活率分别是蔗糖的６倍和麦芽的４．５倍（Ｐ〈０．０１），而且显著高于乳糖（Ｐ〈０．０５）；４种复合保护剂中添加海藻糖后，细胞冻干存活率均显著提高（Ｐ〈０．０１）；海藻糖还可明显改善冻干菌粉的细胞贮藏稳定性，延长活菌保藏期。
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| 关 键 词： | 海藻糖 保护剂 双歧杆菌 真空冷冻干燥 保藏 |
| 文章编号： | 1000-1573(2000)02-0062-04 |
| 修稿时间： | 1999-10-15 |
The initial study on bio-protective role of trehalose during preparation and storage of freeze-dried powder of Bifidobacterium longum |
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| TIAN Hong tao ,ZHANG Chi ,JIA Ying min ,ZHANG Bo lin.The initial study on bio-protective role of trehalose during preparation and storage of freeze-dried powder of Bifidobacterium longum[J].Journal of Agricultural University of Hebei,2000,23(2):62-65. |
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| Authors: | TIAN Hong tao ZHANG Chi JIA Ying min ZHANG Bo lin |
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| Institution: | TIAN Hong-tao
(Department of Fuod Science, Agriculture University of Hehei, Baoding 071001, China)ZHANG Chi
(Institule of Food Science and Technology, China Agriculture University, Beijing lO0094, China)JIA Ying-min
,ZHANG Bo-lin
(Department of Fuod Science, Agriculture University of Hehei, Baoding 071001, China) |
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| Abstract: | Bio-protective role of a new protective additive-trehalose during preparation and storage of freeze-dried powder of Bifidobacterium longum were studied initially. The results indicated that trehalose was most favorable protective additive for the cells survival among four kinds of disaccharide during lyophilization, for instance,the cells survival rate with trehalose was 6 times more than that with surcose;4.5 times more than that with maltose(P<0.01);and significantly higher than that with lactose(P<0.05). When trehalose was added to four kinds of compound pratective additive,the cells freeze-dried survival rate were all greatly raised (P<0.01).The cells stability of freeze-dried powder was improved obviously and the survival period of Bifidobacterium longum was prolonged apparently by trehalose during storage. |
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| Keywords: | trehalose protective additive Bifidobacterium longum lyophilization survival rate storage |
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