In vivo evaluation of gelatin/hyaluronic acid nanofiber as Burn-wound healing and its comparison with ChitoHeal gel |
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| Authors: | Bahman Ebrahimi-Hosseinzadeh Mirsepehr Pedram Ashrafalsadat Hatamian-Zarmi Soheila Salahshour-Kordestani Mahtab Rasti Zahra Beagom Mokhtari-Hosseini Mohammad Mir-Derikvand |
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| Institution: | 1.Department of Clinical Sciences, Faculty of Veterinary Medicine,University of Tehran,Tehran,Iran;2.Department of Biomedical Engineering,Amir Kabir University of Technology, Tehran, Iran and Managing Director,ChitoTech, Tehran,Iran;3.Department of Chemical Engineering, Faculty of Petroleum and Petrochemical Engineering,Hakim Sabzevari University,Sabzevar,Iran;4.Department of Life Sciences Engineering, Faculty of New Sciences and Technologies,University of Tehran,Tehran,Iran |
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| Abstract: | The study aims at performing a comparative assessment of two types of burn wound treatment. The present study was designed to prepare crosslinked and blended two natural polymers nanofiber scaffolds using gelatin (GE) and hyaluronic acid (HA). The GE/HA composite nanofibrous membranes with varied GE/HA weight ratio have also been successfully fabricated by an electrospinning method. The average diameter of GE/HA fibers was in the range of 20 to 150 nm. In vivo efficacy was also investigated based on a deep second degree burns model for Wistar rats. At 14 days post-operation, the dermal defect basically recovered its normal condition. A percentage of wound closure of GE/HA composite nanofibrous membranes and ChitoHeal gel reached up to 81.9 % and 77.8 % respectively, compared with 65 % of the untreated control (p<0.05). Also, histological parameters were assessed on postoperative day 7 and 14. The results of in vivo experiments showed that more epidermis was formed in the gel and scaffold groups compared to the control group. The numbers of inflammatory cells in these two groups were also smaller as compared with the control group, which could well be the reason for the delayed healing in the control group. |
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