| 野桑蚕12S rRNA基因的克隆及序列分析 |
| |
| 引用本文： | 陈淼,王羽骋,郑小坚,薛仁宇.野桑蚕12S rRNA基因的克隆及序列分析[J].江苏蚕业,2005,27(2):8-12. |
| |
| 作者姓名： | 陈淼 王羽骋 郑小坚 薛仁宇 |
| |
| 作者单位： | 苏州大学蚕桑研究所,苏州,215006;江苏省丝绸集团有限公司,南京,210005 |
| |
| 基金项目： | 国家“863”计划资助项目(编号:2004AA2Z1020。) |
| |
| 摘 要： | 通过PCR技术,对中国山东青州野桑蚕mtDNA的12SrRNA基因进行了克隆和序列分析。结果表明,青州野桑蚕12SrRNA基因为788nt,在DNA水平上,与家蚕12SrRNA基因的同源性达到99%,与中国陕西安康、日本筑波来源的野桑蚕12SrRNA基因的同源性分别为97%、98%;分子进化分析显示,家蚕可能是起源于中国野桑蚕。
|
| 关 键 词： | 野桑蚕 家蚕 线粒体 12S rRNA 进化 |
Cloning and Sequence Analysis of the 12S rRNA gene of mtDNA From Chinese Bombyx mandarina M. |
| |
| Chen Mia Zheng Xiaojian Xue Renyu Cao Guangli Gong Chengliang.Cloning and Sequence Analysis of the 12S rRNA gene of mtDNA From Chinese Bombyx mandarina M.[J].Jiangsu Sericulture,2005,27(2):8-12. |
| |
| Authors: | Chen Mia Zheng Xiaojian Xue Renyu Cao Guangli Gong Chengliang |
| |
| Abstract: | 12S rRNA gene of mitochondrial DNA(mtDNA) from the Chinese Qingzhou Bombyx mandarina M. was cloned through PCR. The sequence analysis indicated that the 12S rRNA gene is 788nt in size,and shows 99% similarity with those of mtDNA from different species of B. mori,97% ,and 98% with that of Chinese Ankang and Japanese Tsukuba B. mandarina M on nucleotide level, respectively. Furthermore,the phylogenetic analysis illustrates that Bombyx mori possibly derived from Chinese Bombyx mandarina. |
| |
| Keywords: | Bombyx mandarina M mtDNA 12S rRNA evolution |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
