In vitro effects of dexamethasone on bovine CD25+CD4+ and CD25?CD4+ cells |
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| Institution: | 1. Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell''Università 16, 35020 Legnaro, Padova, Italy;2. Department of Animal Pathology, University of Turin, via L. da Vinci 44, 10095, Grugliasco, Italy;1. LUNAM Université, Oniris, Nantes-Atlantic College of Veterinary Medicine and Food Sciences and Engineering, F-44307 Nantes, France;2. INRA, UMR1300 Biology, Epidemiology and Risk Analysis in Animal Health, F-44307 Nantes, France;3. SCP BOCAVET, F-79140 Cerizay, France;4. ANSES, Bovine Tuberculosis Reference Laboratory, Laboratory for Animal Health, Paris-Est University, F-94706 Maisons-Alfort, France;1. MAEVA SERVET S.L., C/ de la Fragua 3, Alameda del Valle, Madrid 28749, Spain;2. Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Avda, Puerta de Hierro s/n, Madrid 28040, Spain;3. Servicio de Sanidad Animal y Laboratorio del Gobierno de Canarias, San Cristóbal de la Laguna 38320, Spain;4. Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Avda. Puerta de Hierro s/n, Madrid 28040, Spain |
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| Abstract: | This paper investigates the in vitro effect of dexamethasone on bovine CD25highCD4+, CD25lowCD4+ and CD25?CD4+ T cells. Only a small percentage of bovine CD25highCD4+ (2–4%) and CD25lowCD4+ (1–2%) cells expressed Foxp3. Dexamethasone caused considerable loss of CD25?CD4+ cells, but it increased the relative and absolute numbers of CD25highCD4+ and CD25lowCD4+ lymphocytes, while at the same time reducing the percentage of Foxp3+ cells within the latter subpopulations. Considering all these, as well as the intrinsically poor Foxp3 expression in bovine CD25+CD4+, it can be concluded that the drug most probably increased the number of activated non-regulatory CD4+ lymphocytes. It has been found that changes in cell number were at least partly caused by proapoptotic effect of the drug on CD25?CD4+ cells and antiapoptotic effect on CD25highCD4+ and CD25lowCD4+ cells. The results obtained from this study indicate that the involvement of CD4+ lymphocytes in producing the anti-inflammatory and immunosuppressive effect of dexamethasone in cattle results from the fact that the drug had a depressive effect on the production of IFN-γ by CD25?CD4+ cells. Secretion of TGF-β and IL-10 by CD4+ lymphocytes was not involved in producing these pharmacological effects, because the drug did not affect production of TGF-β and, paradoxically, it reduced the percentage of IL-10+CD4+ cells. |
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