The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species |
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| Authors: | Jian-Feng Li Eunsook Park Albrecht G von Arnim Andreas Nebenführ |
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| Institution: | (1) Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville Tennessee, 37996-0840, USA;(2) Current address: Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114-2790, USA |
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| Abstract: | Background Plant genome sequencing has resulted in the identification of a large number of uncharacterized genes. To investigate these
unknown gene functions, several transient transformation systems have been developed as quick and convenient alternatives
to the lengthy transgenic assay. These transient assays include biolistic bombardment, protoplast transfection and Agrobacterium-mediated transient transformation, each having advantages and disadvantages depending on the research purposes. |
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