| 反相高效液相色谱法测定硝碘酚腈含量 |
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| 引用本文： | 葛秀文,陈卫东.反相高效液相色谱法测定硝碘酚腈含量[J].中国兽药杂志,2010,44(8):31-32. |
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| 作者姓名： | 葛秀文 陈卫东 |
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| 作者单位： | 上海中药创新研究中心,上海,201203 |
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| 摘 要： | 采用反相高效液相色谱法(RP-HPLC)测定了硝碘酚腈含量。以乙腈-0.01 mol/L磷酸盐pH6.5(20∶80,V/V)为流动相,Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm),流速1.0 mL/min,柱温25℃,检测波长273 nm。结果表明,在0.050～1.20 mg/mL范围内硝碘酚腈浓度与峰面积呈良好线性关系(r=0.9995),最低检出浓度为2.5 ng/mL,最低定量浓度为5.0 ng/mL。低、中、高3种浓度回收率分别为100.21%、99.07%、101.90%,RSD=0.48%(n=9)。本方法专属性强,准确、简便、灵敏度高,结果稳定,可用于硝碘酚腈的含量测定。
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| 关 键 词： | 硝碘酚腈 反相高效液相色谱法 含量测定 |
| 收稿时间： | 3/5/2010 12:00:00 AM |
| 修稿时间： | 6/4/2010 12:00:00 AM |
Quantity study of Nitroxynil by RP-HPLC |
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| GE Xiu-wen,HUANG Yi-jun,WU Ping,YANG Zi-rong,CHEN Wei-dong.Quantity study of Nitroxynil by RP-HPLC[J].Chinese Journal of Veterinary Drug,2010,44(8):31-32. |
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| Authors: | GE Xiu-wen HUANG Yi-jun WU Ping YANG Zi-rong CHEN Wei-dong |
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| Institution: | Shanghai Innovative Research Center of Traditional Chinese Medicine, |
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| Abstract: | Nitroxynil was determined by a RP-HPLC method.The method was performed with a Agilent Zorbax SB-C18 column（4.6 mm×250 mm,5 μm） at 25 ℃,and the mobile phase was acetonitrile-0.01 mol/L phosphate pH6.5（20∶80,V/V）.The flow rate was 1.0 mL/min.The detection wavelength was 273 nm.The result showed that there was a good linear relationship（r=0.999 5） between peak area and nitroxynil concentration at the range of 0.050～1.20 mg/mL,the limit of detection was 2.5 ng/mL,the limit of quantitation was 5.0 ng/mL.The recovery for low,medium and high concentration was 100.21%,99.07% and 101.90% respectively,RSD was 0.48%（n=9）.The method is selective and simple that suitable for assay of nitroxynil sample. |
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| Keywords: | Nitroxynil RP-HPLC method Assay |
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