| 舟山海域4种鲷科鱼类线粒体Cytb基因全序列克隆分析 |
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| 引用本文： | 徐田军,王日昕,王健鑫.舟山海域4种鲷科鱼类线粒体Cytb基因全序列克隆分析[J].南方水产,2010,6(1):30-36. |
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| 作者姓名： | 徐田军 王日昕 王健鑫 |
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| 作者单位： | 浙江海洋学院海洋科学学院,海洋生物资源与分子工程实验室,浙江,舟山,316000 |
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| 基金项目： | 国家科技支撑计划项目(2007BAD43B08);;浙江省重点科研项目(2007C22041);;浙江省重大科研项目(2007C02001,2007C04302,2008C22026) |
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| 摘 要： | 摘要：克隆测序了分布于舟山海域的真鲷（Pagrus pagrus）、黑鲷（Sparus macrocephalus）、黄鳍棘鲷（Acanthopagrus latus）和二长棘鲷（Parargyrops edita）4种鲷科鱼类的线粒体Cytb基因1141bp的全序列。通过对4种鲷科鱼类Cytb基因序列的比对分析，发现了292个核苷酸变异位点，其中存在186个信息位点，序列中的转换大于颠换，碱基替换多发生于密码子第3位。遗传距离分析表明，4种鲷科鱼类的遗传差异在0．0967～0．2275之间，其中真鲷与二长棘鲷之间遗传距离最小，为0．0967，黄鳍棘鲷与二长棘鲷的遗传距离最大，为0．2275；序列变异的转换／颠换比值在2．2930～7．3587之间。以25种鲈总科鱼类构建的系统树表明鲷科鱼类与其他科的鱼类存在较远的遗传亲缘关系。
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| 关 键 词： | 鲷科 细胞色素b 序列比较 遗传进化 |
Cloning and sequence analysis of Cyt b gene in four Sparidae fishes in Zhoushan sea area |
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| XU Tianjun,WANG Rixin,WANG Jianxin.Cloning and sequence analysis of Cyt b gene in four Sparidae fishes in Zhoushan sea area[J].South China Fisheries Science,2010,6(1):30-36. |
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| Authors: | XU Tianjun WANG Rixin WANG Jianxin |
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| Institution: | Lab. of Marine Living Resources and Molecular Engineering/a>;College of Marine Science/a>;Zhejiang Ocean University/a>;Zhoushan 316000/a>;China |
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| Abstract: | The complete sequences of mitochondrial Cyt b gene in Pagrus pagrus,Sparus macrocephalus,Acanthopagrus latus and Parargyrops edita in Zhoushan sea area were amplified,cloned and sequenced.The 1 141 bp Cyt b gene sequences of these four species were obtained.Compared with the same DNA fragment recorded in the GeneBank,the sequences of 1 141 bp DNA had 292 nucleotide variation sites,among which 186 parsimony informative sites were observed.Transitions were discovered to be more than transversions,and most of ... |
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| Keywords: | Spridae Cyt b sequence analysis genetic evolution |
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