| 德氏假单胞菌(Pseudomonas delafieldii)R-8脱硫基因启动子的克隆与鉴定 |
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| 引用本文： | 余志坚,李焕杰,王海胜,李信.德氏假单胞菌(Pseudomonas delafieldii)R-8脱硫基因启动子的克隆与鉴定[J].江苏农业学报,2009,25(3). |
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| 作者姓名： | 余志坚 李焕杰 王海胜 李信 |
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| 作者单位： | 1. 中国农业科学院研究生院生物化学与分子生物学实验室,北京100081;东华理工大学生物系,江西抚州344000
2. 中国农业科学院研究生院生物化学与分子生物学实验室,北京,100081 |
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| 基金项目： | 国家"863"计划项目 |
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| 摘 要： | 利用PCR方法,从专一性脱硫菌德氏假单胞菌(Pseudomonas delafieldii)R-8中克隆了脱硫基因启动子系列缩短的片段,连接至启动子探测型表达载体pPR9TT,电转原始菌R-8,并测定重组菌R-8-P中的报告基因LacZ表达量.结果表明脱硫基因核心启动子区缩短至300 bp,并对启动子序列进行了预测.
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| 关 键 词： | 德氏假单胞菌 脱硫基因 启动子 |
Cloning and Characterization of the dsz Promoter Region from Pseudomonas delafieldii R-8 |
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| YU Zhi-jian,LI Huan-jie,WANG Hai-sheng,LI Xin.Cloning and Characterization of the dsz Promoter Region from Pseudomonas delafieldii R-8[J].Jiangsu Journal of Agricultural Sciences,2009,25(3). |
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| Authors: | YU Zhi-jian LI Huan-jie WANG Hai-sheng LI Xin |
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| Institution: | 1.Laboratory of Biochemical and Molecular Biology;Graduate School of Chinese Academy of Agricultural Sciences;Beijing 100081;China;2.Department of Biology;East China Institute of Technology;Fuzhou 344000;China |
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| Abstract: | The dsz promoter serial deletion fragments were cloned by PCR from strain Pseudomonas delafieldii R-8 which can convert dibenzothiophene into 2-hydroxybiphenyl and sulfate.Series fragments were cloned into the detection of expression vector pPR9TT,then reintroduced into strain R-8 to obtain engineering strains R-8-P,and the expression of reporter gene,LacZ,in R-8-P was determined.The results showed that the size of the dsz core promoter region was 300 bp,and regulation region was forecasted. |
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| Keywords: | Pseudomonas delafieldii R-8 desulfurization gene promoter pPR9TT |
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