| 红曲菌T-DNA插入突变库转化子的特征及代谢产物分析 |
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| 引用本文： | 徐驰,邵彦春,吴佳佳,叶阳,李利,李琦,陈福生.红曲菌T-DNA插入突变库转化子的特征及代谢产物分析[J].华中农业大学学报,2009,28(2). |
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| 作者姓名： | 徐驰 邵彦春 吴佳佳 叶阳 李利 李琦 陈福生 |
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| 作者单位： | 华中农业大学食品科技学院/农业部食品安全评价重点开放实验室/农业微生物学国家重点实验室,武汉,430070 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，教育部人才计划项目 |
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| 摘 要： | 以农杆菌介导的红曲菌T-DNA插入突变库中8 000多株转化子为材料,通过菌落形态观察,获得230株形态变化显著的突变子,并将其分为7类.在此基础上,分析了14株代表菌株产色素、莫纳可啉K(Mo-nacolin K)、桔霉素和淀粉酶的情况.结果表明:所有供试菌株的产色素能力均低于出发菌株M-7,其中806#和2553#的色价均不到M-7的1/10;10株突变菌株产Monacolin K的能力高于M-7,其中3245#产Monacolin K的能力较M-7提高了近10倍,达到679.25μg/g;1822#产桔霉素(干红曲)能力最高,达60.01 ng/g,是M-7的近30倍,而806#和178#产桔霉素能力较低,在试验条件下未检测到;13株突菌株的淀粉酶活高于M-7,1067#最高约为M-7的20倍,达3 227.10 U/g.
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| 关 键 词： | 红曲菌 转化 形态 代谢产物 |
Evaluation of T-DNA Insertional Library of Monascus Mediated by Agrobacterium tume f aciens |
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| XU Chi,SHAO Yan-chun,WU Jia-ji,YE Yang,LI Li,LI Qi,CHEN Fu-sheng.Evaluation of T-DNA Insertional Library of Monascus Mediated by Agrobacterium tume f aciens[J].Journal of Huazhong Agricultural University,2009,28(2). |
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| Authors: | XU Chi SHAO Yan-chun WU Jia-ji YE Yang LI Li LI Qi CHEN Fu-sheng |
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| Institution: | College of Food Science and Technology;Huazhong Agriultural University/Key Laboratory of Food Safety of Ministry of Agriculture/State Key Laboratory of Agricultural Microbiology;Wuhan 430070;China |
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| Abstract: | About 8 000 transformants were obtained by T-DNA insertional library of Monascus ruber mediated by Agrobacterium tumefaciens.230 of them with significant changes were classified into seven groups according to characteristics of the colony and micro-morphological observation.On this basis,pigment,Monacolin K,citrinin and amylase of the representatives of the14 mutants were analyzed.The results were as follows: the capacity to produce pigment of all the mutants were poorer than the original strain M-7;806# an... |
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| Keywords: | Monascus spp transformation morpha metabolite |
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