| 东方百合花器官组织培养研究 |
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| 引用本文： | 赵得萍,唐道城,刘米会,郭志.东方百合花器官组织培养研究[J].北方园艺,2008(2):198-200. |
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| 作者姓名： | 赵得萍 唐道城 刘米会 郭志 |
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| 作者单位： | 青海大学,高原花卉研究中心,青海,西宁,810016 |
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| 基金项目： | 国家科技部农业科技成果转化资助项目 |
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| 摘 要： | 取东方百合Sorbonne的花萼、花瓣和花丝为外植体,接种于经过筛选的最佳诱导培养基上,进行诱导率和分化率的比较,将花丝分成3段,进行分段培养的最佳培养基和最佳花丝部位筛选.结果表明:花萼的愈伤诱导率和不定芽再分化率最高,分别为92.0%和89%,花瓣次之,花丝最低.花丝分段培养的最佳诱导培养基为MS NAA 1.0 mg/L BA 0.2 mg/L;花丝各部位分化能力是中部＞基部＞顶部;中部花丝在 MS NAA 1.0 mg/L BA 0.2 mg/L培养基上的愈伤诱导率和不定芽再分化率都达到100%.
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| 关 键 词： | 东方百合 组织培养 花萼 花瓣 花丝 |
| 文章编号： | 1001-0009（2008）02-0198-03 |
| 收稿时间： | 2007-09-13 |
| 修稿时间： | 2007年9月13日 |
Study on Floral Organ Culture of Oriental Lily |
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| ZHAO De-ping,TANG Dao-cheng,LIU Mi-hui,GUO Zhi.Study on Floral Organ Culture of Oriental Lily[J].Northern Horticulture,2008(2):198-200. |
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| Authors: | ZHAO De-ping TANG Dao-cheng LIU Mi-hui GUO Zhi |
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| Abstract: | The calyxes,petals and filaments of Sorbonne were explanted respectively on best medium which had selected to compare the frequency of callus initiation and buds regeneration,the filament was divided up three sections to select their preferable initiation medium and preferable position of filament.The results showed that,the calyx had high frequency of callus initiation and bud regeneration,with 92.0% and 89% respectively,petal was in second place and filament was the last one.The best callus initiation medium for divided filament culture was MS NAA1.0 mg/L BA0.2 mg/L;The callus initiation of different filament sections was different,the sequence was central section>basal section>top section;the frequency of callus initiation and bud regeneration of central section filament explanted on MS NAA 1.0 mg/L BA 0.2 mg/L was 100% either. |
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| Keywords: | Oriental Lily Tissue Culture Calyxes Petals Filaments |
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