Interferon-gamma response of PBMC indicates productive pseudorabies virus (PRV) infection in swine |
| |
| Authors: | Hoegen Bärbel Saalmüller Armin Röttgen Marlene Rziha Hanns-Joachim Geldermann Hermann Reiner Gerald Pfaff Eberhard Büttner Mathias |
| |
| Institution: | Federal Research Centre for Virus Diseases of Animals, Institute of Immunology, Paul-Ehrlich-Strasse 28, D72076 Tübingen, Germany. |
| |
| Abstract: | In Chinese Meishan/German Landrace cross-bred swine F2 generation interferon gamma (IFN-gamma) production by peripheral blood mononuclear cells (PBMC) was determined directly ex vivo at different time points after survival of a virulent pseudorabies virus (PRV) infection. This reactivity was compared with the reactivity of na?ve PBMC. Significant IFN-gamma production was determined in ELISA and ELISPOT only after in vitro PBMC re-stimulation with PRV and not with the closely related bovine herpesvirus BHV-1. The PRV-specific IFN-gamma secretion from re-stimulated PBMC showed high levels 6 days after infection, before the presence of serum antibodies, and it persisted at a high level over a 3 months period. The response of a group of eight piglets infected intranasally with PRV varied. Only two animals showed the expected typical fever response. PRV specific IFN-gamma production by PBMC clearly indicated that infection had occurred. Early significant IFN-gamma production by primed PBMC turned out to be a reliable and specific ex vivo marker for cellular response against productive PRV infection in swine before antibody formation. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
