| 消毒方法对红花种子发芽率的影响和种子环境细菌研究 |
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| 引用本文： | 马梦雪,赵玲玲,唐思,陈贤军,覃瑞.消毒方法对红花种子发芽率的影响和种子环境细菌研究[J].作物杂志,2018,34(6):162-3591. |
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| 作者姓名： | 马梦雪 赵玲玲 唐思 陈贤军 覃瑞 |
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| 作者单位： | 中南民族大学生命科学学院/武陵山区特色资源植物种质保护与利用湖北重点实验室,430074,湖北武汉 |
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| 基金项目： | 湖北省科技条件平台建设专项(2017BE014);湖北省技术创新专项重大项目(2018ABA093) |
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| 摘 要： | 红花（Carthamus tinotorius L.）种子在发芽和组培中染菌情况严重,发芽率较低,针对这一现象,对供试红花种子进行消毒以降低染菌率并提高发芽率。选择种子预浸泡时间、消毒剂种类、消毒时间3个因素,采用L9（3 4）正交试验,以种子的发芽率、发芽势和染菌率为考察指标,综合分析种子消毒效果。结果表明,消毒剂种类是影响红花种子消毒效果的最重要因素。红花种子最佳消毒方法为种子消毒前用无菌水预浸泡12h,再经2% NaClO消毒10min,染菌率仅为8%,发芽率高达90.67%。另外,对分离纯化消毒后种子所带的细菌16S rDNA（GenBank登录号：MH190221）进行了PCR扩增,序列Blast结果显示,该菌为阿耶波多芽孢杆菌（Bacillus aryabhatti）,初步判断该菌可能为红花种子内生菌。该研究结果为快速获得生长良好的无菌苗、后续的组织培养以及遗传转化奠定了基础。
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| 关 键 词： | 红花 种子消毒 发芽率 正交试验 细菌 |
| 收稿时间： | 2018-05-09 |
The Effects of Different Disinfection Methods on Seed Germination and Study on the Environmental Bacteria in Safflower (Carthamus tinctorius L.) |
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| Ma Mengxue,Zhao Lingling,Tang Si,Chen Xianjun,Qin Rui.The Effects of Different Disinfection Methods on Seed Germination and Study on the Environmental Bacteria in Safflower (Carthamus tinctorius L.)[J].Crops,2018,34(6):162-3591. |
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| Authors: | Ma Mengxue Zhao Lingling Tang Si Chen Xianjun Qin Rui |
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| Institution: | College of Life Sciences, South-Central University for Nationalities/Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China, Wuhan 430074, Hubei, China |
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| Abstract: | Contamination was a serious problem in seed germination and tissue culture of safflower (Carthamus tinctorius L.). In our study, seeds were disinfected in order to reduce the contamination rate.Three factors including soaking time, disinfecting agents and disinfecting time were used for L9(3 4) orthogonal experiment. Results were comprehensively evaluated by using the weighted scoring method based on indexes such as germination rate, germination vigor and contamination rate. Orthogonal test showed that the disinfecting agent was the most important factor affecting the seeds germination. Among various treatments, seeds soaked in sterile water for 12h followed by the 2% sodium hypochlorite (NaClO) for 10min was the best disinfecting method in safflower seeds which yield a contamination rate of 8% and a germination rate of 90.67%. In addition, 16S rDNA (GenBank Accession No. MH190221) of a bacterium was isolated from seeds and purified after disinfection which was amplified by PCR. BLAST results showed that it belonged to Bacillus aryabhatti which was most likely to be entophytes in safflower seeds. The results of this study provided an important basis for obtaining well-grown aseptic seedlings, tissue culture as well as genetic transformation. |
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| Keywords: | Carthamus tinctorius L Seed disinfection Seed germination rate Orthogonal experiment Bacteria |
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