| 一株甘蔗内生菌鉴定及其溶磷能力的研究 |
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| 引用本文： | 狄义宁,刘鲁峰,谢林艳,李咏梅,何鹏飞,崔文艳,李富生,何丽莲.一株甘蔗内生菌鉴定及其溶磷能力的研究[J].作物杂志,2018,34(6):68-111. |
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| 作者姓名： | 狄义宁 刘鲁峰 谢林艳 李咏梅 何鹏飞 崔文艳 李富生 何丽莲 |
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| 作者单位： | 1 云南农业大学农学与生物技术学院,650201,云南昆明2 云南农业大学植物保护学院,650201,云南昆明3 云南农业大学甘蔗研究所,650201,云南昆明 |
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| 基金项目： | 云南省现代农业甘蔗产业技术体系建设专项(2017-2018);福建农林大学国家甘蔗工程技术研究中心开放课题(NER02018.6.1) |
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| 摘 要： | 采用形态观察、生理生化测定以及16S rDNA、gyrB和rpoB基因片段比对,对分离自甘蔗品种粤糖86-368茎部内的一株D5内生菌进行分类鉴定。在此基础上,使用平板溶磷圈法和液体培养法研究其溶磷能力,并通过盆栽试验探究其对玉米幼苗生长的影响。结果表明：D5菌株属于Pseudomonas extremorientalis;D5菌株对不同磷酸盐的溶磷能力表现不同,表现为磷酸三钙>植酸钙>磷酸铁,溶磷量分别为0.939±0.012、0.655±0.016、0.125±0.005mg/mL,同时,在溶磷过程中,液体培养基的pH均有不同程度的降低,活菌数除了在磷酸铁培养基中一直下降外,其他磷源培养基中均是先升后降的趋势。施用菌剂后,处理组（D5+P）比对照组（CK+P）玉米幼苗的总鲜重、总干重、苗株总磷含量分别增加45.92%、32.65%、45.01%。因此,D5菌株具有制备微生物菌肥的潜力。
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| 关 键 词： | 甘蔗 内生菌 分子鉴定 溶磷 |
| 收稿时间： | 2018-06-22 |
Identification and Characterization of a Phosphate-Solubilizing Endophyte from Sugarcane |
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| Di Yining,Liu Lufeng,Xie Linyan,Li Yongmei,He Pengfei,Cui Wenyan,Li Fusheng,He Lilian.Identification and Characterization of a Phosphate-Solubilizing Endophyte from Sugarcane[J].Crops,2018,34(6):68-111. |
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| Authors: | Di Yining Liu Lufeng Xie Linyan Li Yongmei He Pengfei Cui Wenyan Li Fusheng He Lilian |
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| Institution: | 1 College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan,China2 College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan, China3 Sugarcane Research Institute, Yunnan Agricultural University, Kunming 650201, Yunnan, China |
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| Abstract: | An endophytic D5 strain was isolated from the stem of Yuetang 86-368 which came from one of the sugarcane cultivars.Colony morphology characteristics, physiological and biochemical tests, 16S rDNA, gyrB and rpoB sequence analyses were carried out. Phosphate-solubilizing capability of the strain was studied by methods of phosphorus hole and liquid culture, and the effect on the growth of maize seedling was studied by a pot experiment. The results showed that the D5 strain belonged to Pseudomonas extremorientalis. Its phosphate-solubilizing capability to different phosphate was different which followed the trend: tricalcium phosphate > phytic acid calcium > iron phosphate. The phosphate-solubilizing capacity was 0.939±0.012, 0.655±0.016, 0.125±0.005mg/mL, respectively. The pH value of the liquid media decreased to different extents and the numbers of living bacterium followed a trend of initial rise and then fall in all phosphorus source in the culture medium except in the iron phosphate cultivation showing a straight decline of dissolved phosphorus. Compared with the control (CK+P), the total fresh weight, total dry weight and total phosphorus content of maize seedlings in the D5 strain were increased by 45.92%, 32.65% and 45.01%, respectively. Therefore, it is an excellent candidate for microbial fertilizer. |
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| Keywords: | Sugarcane Endophyte Molecular identification Phosphate solubilizing |
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