| 应用Nested—PCR技术检测人工感染绵羊蓝舌病病毒 |
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| 引用本文： | 马洪超,孙淑芳,陶茂晖,尹燕博,郑文伯,蒋正军,蔡丽娟.应用Nested—PCR技术检测人工感染绵羊蓝舌病病毒[J].中国动物检疫,2000,17(12):20-22. |
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| 作者姓名： | 马洪超 孙淑芳 陶茂晖 尹燕博 郑文伯 蒋正军 蔡丽娟 |
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| 作者单位： | [1]农业部动物检疫所，青岛266032 [2]河北畜牧兽医总站 |
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| 摘 要： | 实验采用6只绵羊分成3组，一组作为对照接种BHK21细胞上清液，另二组分别接种BIV10和中国分离株Z1株，攻毒定期采血提取抗原并提取病毒RNA，同时进行琼脂扩散试验、普通PCR、Nested-PCR试验。结果在整个攻毒期间，琼脂扩散方法检测不到抗原，普通PCR方法只能在攻毒后12d-15d测得抗原，而Nested-PCR在攻毒后6d即可测得病毒RNA，并且可一直持续到30d。试验证明，Nested-PCR方法是高度敏感的检测抗原的方法。在临床上能够较早地检出抗原，这对于及时采取有效措施，控制疾病流行具有积极的意义。
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| 关 键 词： | Nested－PCR技术 检测 人工感染 绵羊 蓝舌病病毒 琼脂扩散试验 |
Application of Nested - PCR Technique for the Detection of BTV Antigen in Serum Samples From Experimentally Infected Sheep |
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| Ma HongChao,Sun ShuFang,Tao MaoHui,Yin YanBao,Zheng WenBo,Jiang ZhengJun,Cai LiJuan.Application of Nested - PCR Technique for the Detection of BTV Antigen in Serum Samples From Experimentally Infected Sheep[J].China Journal Of Animal Quarantine,2000,17(12):20-22. |
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| Authors: | Ma HongChao Sun ShuFang Tao MaoHui Yin YanBao Zheng WenBo Jiang ZhengJun Cai LiJuan |
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| Abstract: | sheep were divided into 3 groups. The first group was inoculated with BTV10, the second group with Z1(BTV16) a Chinese local strain, the third group with BHK21 cell tissue culture supernatant as control. Blood samples were collected regularly after inoculation. BTV antigen BTV RMA were extracted from blood. AGID can not defect BTV antigen during the entire experiment. PCR can only detect antigen at 15dpi. Nested - PCR can detect BTV RNA from 6 to 30dpi.The experiment proved that Nested-PCRis a highly sensitive technique for detecting BTV antigen. It can detect BTV antigen earlier on clinical samples. It's important for early detection and prevention of BTV disease transmission. |
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| Keywords: | BTV Antigen Nested - PCR AG |
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