| 2种野外采集白腐真菌分离及其产漆酶液体培养基优化 |
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| 引用本文： | 李环明,杨洪升,薛春梅.2种野外采集白腐真菌分离及其产漆酶液体培养基优化[J].安徽农业科学,2017,45(3). |
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| 作者姓名： | 李环明 杨洪升 薛春梅 |
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| 作者单位： | 佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯154007;佳木斯大学应用微生物研究所,黑龙江佳木斯154007 |
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| 基金项目： | 黑龙江省教育厅科学技术研究面上项目，佳木斯大学研究生科技创新项目 |
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| 摘 要： | 目的]优化2种野外采集白腐真菌菌丝产漆酶液体培养基。方法]对2种采集的白腐真菌轮纹韧革菌(Stereum ostrea)和朱红密孔菌(Pycnoporus cinnabarinus)通过分离纯化得到白腐真菌菌丝,应用ABTS法测定其产漆酶活性,研究碳源、氮源、金属阳离子、p H、温度、转速对白腐真菌产漆酶的影响。结果]轮纹韧革菌最佳培养基是葡萄糖20.00 g、硝酸钾5.00 g、NaH_2PO_45.00 g、Cu SO41.00 g、初始p H 6、温度25℃、转速170 r/min,在第11天其产漆酶活性达到最大,为1.557 U/m L,是PDA液体培养基产漆酶活性的2.7倍。朱红密孔菌最佳培养基是麦芽糖25.00 g、酵母膏5.00 g、NaH_2PO_41.00 g、KCl 2.00 g、初始p H 7、温度30℃、转速200 r/min,在第9天其产漆酶活性达到最大,为1.478 U/m L,是PDA液体培养基产漆酶活性的2.3倍。结论]筛选出2种野外白腐真菌产漆酶最佳培养基,为漆酶的进一步研究提供了参考。
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| 关 键 词： | 白腐真菌 漆酶 培养基 筛选 |
Isolation of Two Species of Field Acquisition White Rot Fungi and Optimization of Liquid Culture Medium of Their Produce Laccase |
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| LI Huan-ming,YANG Hong-sheng,XUE Chun-mei.Isolation of Two Species of Field Acquisition White Rot Fungi and Optimization of Liquid Culture Medium of Their Produce Laccase[J].Journal of Anhui Agricultural Sciences,2017,45(3). |
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| Authors: | LI Huan-ming YANG Hong-sheng XUE Chun-mei |
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| Abstract: | Objective] The aim was to optimize the liquid culture medium of two species of field acquisition white rot fungi. Method] By isola-ting and purifying two species of white rot fungi,Stereum ostrea and Pycnoporus cinnabarinus,white rot fungus hyphae were collected, and the liq-uid culture medium of laccase was screened.ABTS method was applied to measure the activity of laccase.Effects of carbon sources,nitrogen sources,metal cations,pH,temperature and rotational speed on laccase produced by white rot fungi were studied.Result] The optimum medium of Stereum ostrea was glucose 20.00 g,potassium nitrate 5.00 g,NaH2 PO45.00 g,CuSO41.00 g,the initial pH 6,temperature 25 ℃, rotational speed 170 r/min,and the laccase activity reached the maximum on the 11th day the maximum value reached 1.557 U/mL which was 2.7 times of the laccase activity of PDA liquid culture.The optimum medium of Pycnoporus cinnabarinus was maltose 25.00 g,yeast extract 5.00 g, NaH2 PO41.00 g,KCl 2.00 g,the initial pH 7,temperature 30 ℃,rotational speed 200 r/min,and the laccase activity reached the maximum in the 9 d,the maximum value 1.478 U/mL which was 2.3 times of the laccase activity of PDA liquid culture.Conclusion] The opitimal liquid cul-ture medium of two species of field acquisition white rot fungi is obtained so as to provide reference for further study of laccase. |
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| Keywords: | White rot fungus Laccase Culture medium Screen |
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