| 犬贾第虫病毒(长春株)全基因组序列分析 |
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| 引用本文： | 陈丽凤,李建华,张西臣,刘全,赵月萍,曹利利,陈超.犬贾第虫病毒(长春株)全基因组序列分析[J].畜牧兽医学报,2006,37(4):408-411. |
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| 作者姓名： | 陈丽凤 李建华 张西臣 刘全 赵月萍 曹利利 陈超 |
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| 作者单位： | 1. 吉林大学畜牧兽医学院,长春,130062
2. 军事医学科学院军事兽医研究所,长春,130062 |
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| 基金项目： | 国家自然科学基金（30300260） |
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| 摘 要： | 根据已报道的人源蓝氏贾第虫病毒序列设计了互相重叠的6对特异性引物，以犬贾第虫（长春株）纯培养滋养体总核酸为模板进行RT—PCR。PCR产物连接到pMD18-T载体进行测序，通过BLAST在GenBank进行同源性搜索，并用DNAMAN分子生物学软件进行分析。结果测得我国犬贾第虫病毒（长春株）基因组全长为6276bp（DQ238861），编码1个有887个氨基酸残基的衣壳蛋白和1056个氨基酸残基的融合蛋白，这2个阅读框被-1核糖体移码框分开，重叠处有220nt。基因组中G＋C占49．62％。其序列与国外报道的人源蓝氏贾第虫病毒（L13218）序列同源性为94．62％，编码的氨基酸同源性为93．50％；与国内人源蓝氏贾第虫病毒（AF525216）序列同源性为98．88％，编码的氨基酸同源性为98．30％。
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| 关 键 词： | 犬贾第虫病毒 全长cDNA序列 基因组 序列分析 |
| 文章编号： | 0366-6964（2006）04-0408-04 |
| 收稿时间： | 2005-03-30 |
| 修稿时间： | 2005-03-30 |
The Analysis of Full-length cDNA Sequence of Giardia canis Virus Changchun Strain |
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| CHEN Li-feng,LI Jian-hua,ZHANG Xi-chen,LIU Quan,ZHAO Yue-ping,CAO Li-li,CHEN Chao.The Analysis of Full-length cDNA Sequence of Giardia canis Virus Changchun Strain[J].Acta Veterinaria et Zootechnica Sinica,2006,37(4):408-411. |
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| Authors: | CHEN Li-feng LI Jian-hua ZHANG Xi-chen LIU Quan ZHAO Yue-ping CAO Li-li CHEN Chao |
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| Institution: | 1. College of Animal Husbandry and Veterinary Medicine, Jilin University, Changchun 130062, China;2. Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130062 ,China |
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| Abstract: | According to the published nucleotide sequence of giardiavirus,six pairs of primers were designed and total nucleic acids from trophozoites of Giardia canis isolated from dogs in Changchun were used as template for RT-PCR.After RT-PCR,the products were linked into pMD18-T vector,then cloned,sequenced and analyzed.The genome sequence of Giardia canis virus was 6 276bp(DQ238861),which revealed the presence of two large open reading frames that are separated by a-1 frameshift and share an overlap of 220 nt.The ORF1(367-3030) encodes a polypeptide of 887 amino acid residues.The ORF2(2808-5981) encodes a polypeptide of 1 056 amino acid residues,which contains all consensus RNA-dependent RNA polymerase sequence motifs.The ratio of G C in the genome was 49.62%.The homology of Giardia canis virus to sequence of L13218(GenBank) was 94.62% in the nucleotide,and 93.50% in the amino acid levels.Compared with the sequences of AF525216(GenBank),the homology was 98.88% in the nucleotide,and 98.30% in the amino acid levels. |
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| Keywords: | Giardia canis virus full-length cDNA sequence genome sequence analysis |
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