Differential effects of phytotoxic metabolites from <Emphasis Type="Italic">Alternaria tagetica</Emphasis> on <Emphasis Type="Italic">Tagetes erecta</Emphasis> cell cultures |
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| Authors: | Joaquin A Qui Lizbeth A Castro-Concha Karlina García-Sosa Luis Manuel Peña-Rodríguez María L Miranda-Ham |
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| Institution: | 1.Unidad de Bioquímica y Biología Molecular de Plantas,Centro de Investigación Científica de Yucatán,Mérida,Mexico;2.Unidad de Biotecnología,Centro de Investigación Científica de Yucatán,Mérida,Mexico |
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| Abstract: | Alternaria tagetica, a fungus that causes early blight in marigold (Tagetes erecta), produces two groups of phytotoxic metabolites: one hydrophilic and the other lipophilic that show phytotoxic activity when
tested by the leaf-spot assay in T. erecta. We evaluated the cellular effects of the phytotoxic culture filtrate of A. tagetica and the hydrophilic and lipophilic fractions, then determined whether the filtrate or the fractions differentially induced
pathogenesis-related mechanisms in the plant. The culture filtrate and the phytotoxic fractions had adverse effects on cell
viability, fresh mass, and the number of cells, and induced ROS accumulation, lipid peroxidation and DNA damage on T. erecta cell suspension cultures, and these effects are related to pathogenic mechanisms attributed to phytotoxins. However, although
exposure of marigold cells to the phytotoxic culture filtrate of A. tagetica triggered programmed cell death, the hydrophilic and the lipophilic phytotoxic fractions induced death that was more related
to a toxic effect leading to necrosis. This study presents a complementary perspective in the search for the roles of metabolites,
including phytotoxins, produced by phytopathogenic fungi during plant infection. |
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