| 小麦蓝矮病植原体16S rDNA序列分析研究 |
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| 引用本文： | 张荣,孙广宇,张雅梅,康振生.小麦蓝矮病植原体16S rDNA序列分析研究[J].植物病理学报,2005,35(5):397-402. |
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| 作者姓名： | 张荣 孙广宇 张雅梅 康振生 |
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| 作者单位： | 西北农林科技大学植保学院, 陕西杨凌 712100 |
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| 基金项目： | 陕西省自然科学基金，西北农林科技大学校科研和教改项目 |
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| 摘 要： | 小麦蓝矮病是我国西北地区冬小麦上一种重要病害。本研究利用植原体16S rDNA通用引物对小麦蓝矮病患病植株全DNA进行nest-PCR扩增,获得1.2 kb的特异片段,并对扩增产物进行核苷酸序列测定,从分子水平证明了小麦蓝矮病的病原是植原体。利用最大简约法构建了16S rDNA系统演化树,系统演化关系分析表明:小麦蓝矮病植原体应该归属于翠菊植原体(Candidatus Phytoplasma asteris);小麦蓝矮病植原体与三叶草变叶病植原体(CPh)关系密切,被聚类为同一亚组(16Sr I-C),但是它们在寄主范围和传播介体等生物学性状方面差异很大。
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| 关 键 词： | nested-PCR 小麦 三叶草变叶病植原体 系统发育 小麦蓝矮病 |
| 文章编号： | 0412-0914(2005)05-0397-06 |
| 收稿时间： | 2005-01-04 |
| 修稿时间： | 2005年1月4日 |
Sequence analysis of 16S ribosomal DNA of phytoplasma associated with wheat blue dwarf |
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| ZHANG Rong,SUN Guang-yu,ZHANG Ya-mei,KANG Zhen-sheng.Sequence analysis of 16S ribosomal DNA of phytoplasma associated with wheat blue dwarf[J].Acta Phytopathologica Sinica,2005,35(5):397-402. |
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| Authors: | ZHANG Rong SUN Guang-yu ZHANG Ya-mei KANG Zhen-sheng |
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| Institution: | College of Plant Protection, Northwest A & F University, Yangling Shaanxi 712100, China |
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| Abstract: | Wheat blue dwarf is an important disease on winterwheat in northwestern China.A 1.2 kb fragmentwas amplified by nested-PCR phytoplasma universal primers and total DNA sample extracted from d iseased wheat as the amplified template.Nucleotide sequencing of the amplified fragment ind icated thatwheat blue dwarf d isease was caused by a pathogenic phytoplasma.A phylogenetic tree based on 16S ribosomal DNA sequences was con-structed and showed that wheat blue dwarf phytoplasma was clustered into theCandidatusPhytoplasma asteris,subgroup 16Sr I-C.Wheat blue dwarf phytoplasma and clover phyllody phytoplasma had a high sim ilarity on 16S rDNA sequences,but d iffered in host range and d issem ination agent. |
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| Keywords: | nested-PCR |
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