| 香椿叶提取物清除DPPH自由基能力的测定方法 |
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| 引用本文： | 陈丛瑾,黄克瀛,李德良,王旭强,袁双山.香椿叶提取物清除DPPH自由基能力的测定方法[J].林产化学与工业,2006,26(3):69-72. |
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| 作者姓名： | 陈丛瑾 黄克瀛 李德良 王旭强 袁双山 |
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| 作者单位： | 1. 中南林业科技大学,工业学院,湖南,株洲,412006;广西大学,化学化工学院,广西,南宁,530004
2. 中南林业科技大学,工业学院,湖南,株洲,412006 |
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| 摘 要： | 建立了以分光光度法测定天然抗氧化剂清除2,2-二苯基-1-苦肼基(DPPH)自由基能力的方法。通过测定芦丁、檞皮素、抗坏血酸、没食子酸的DPPH自由基清除率曲线,提出以IC50值作为评价试样清除DPPH自由基能力的指标,并将此应用于测定香椿叶提取物清除DPPH自由基的能力。通过测定香椿叶提取物与DPPH溶液反应后,DPPH溶液在517 nm处的吸光度的变化,确定了测定香椿叶提取物清除DPPH自由基能力的条件。即:测定波长517 nm,反应时间50 m in。在此条件下测得的以总黄酮计的香椿叶提取物清除DPPH自由基的IC50值为22.026。
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| 关 键 词： | 香椿叶提取物 分光光度法 清除能力 二苯基苦肼基 |
| 文章编号： | 0253-2417（2006）03-0069-04 |
| 收稿时间： | 09 30 2005 12:00AM |
| 修稿时间： | 2005-09-30 |
Determining Method of DPPH Free Radical Scavenging Activity of Toona sinensis (A.Juss.) Roem. Leaves Extracts |
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| CHEN Cong-jin,HUANG Ke-ying,LI De-liang,WANG Xu-qiang,YUAN Shuang-shan.Determining Method of DPPH Free Radical Scavenging Activity of Toona sinensis (A.Juss.) Roem. Leaves Extracts[J].Chemistry & Industry of Forest Products,2006,26(3):69-72. |
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| Authors: | CHEN Cong-jin HUANG Ke-ying LI De-liang WANG Xu-qiang YUAN Shuang-shan |
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| Institution: | 1. Industry College, Central South University of Forestry and Technology, Zhuzhou 412006, China; 2. Chemistry and Chemical Engineering College,Guangxi University, Nanning 530004, China |
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| Abstract: | A spectrophotometric method was established for determining 2,2-diphenyl-1-picrylhydrazyl(DPPH)free radical(scavenging) activity of natural antioxidants.The evaluating criterion of DPPH free radical(scavenging) activity was regarded as("IC_(50)")by determining the scavenging rate curves of rutin,quercetin,ascorbic acid and gallic acid.The DPPH free radical(scavenging) activity of extract from Toona sinensis(A.Juss.)Roem.leaves was expressed by IC_(50).The condition of scavenging DPPH free radical of extracts from T.sinensis.leaves was determined by change of absorbency veriety at wavelength 517 nm after reacting DPPH with extracts.It was wavelength 517,reacting time 50 min.Under this condition,IC_(50) of scavenging DPPH free radical of extracts from T.sinensis leaves is 22.026. |
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| Keywords: | Toona sinensis(A Juss )Roem leaves extracts spectrophotometry scavenging activity 2 2-diphenyl-1-picrylhydrazyl |
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