| 柱前衍生高效液相色谱法测定白菜及小麦中草甘膦残留量 |
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| 引用本文： | 马为民,林小虎,张燕,张珊珊,周印富.柱前衍生高效液相色谱法测定白菜及小麦中草甘膦残留量[J].北方园艺,2008(6). |
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| 作者姓名： | 马为民 林小虎 张燕 张珊珊 周印富 |
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| 作者单位： | 河北科技师范学院,河北,秦皇岛,066004 |
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| 基金项目： | 国家科技支撑计划重大项目 |
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| 摘 要： | 运用荧光衍生试剂FMOC-Cl衍生草甘膦后,利用双聚合氨基柱HPLC测定了白菜及小麦中草甘膦的含量,该方法灵敏度高,可靠性强.通过试验,找到了最佳衍生条件和色谱条件.该方法通过测定蔬菜及小麦样品得到了确证,在添加草甘膦标样1 mg/kg时,最低检出限为0.3 mg/kg,平均回收率为82.4%,变异系数为10.3%.
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| 关 键 词： | 草甘膦 高效液相色谱法 聚合氨基柱 柱前衍生 |
HPLC Method for Determination of Glyphosate on Chinese Cabbage and Wheat |
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| MA Wei-min,LIN Xiao-hu,ZHANG Yan,ZHANG Shan-shan,ZHOU Yin-fu.HPLC Method for Determination of Glyphosate on Chinese Cabbage and Wheat[J].Northern Horticulture,2008(6). |
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| Authors: | MA Wei-min LIN Xiao-hu ZHANG Yan ZHANG Shan-shan ZHOU Yin-fu |
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| Abstract: | This paper described a robust and sensitive HPLC method for determination of glyphosate on vegetable material after pre-derivatisation with 9-fluorenylmethyl chloroformate(FMOC-Cl) using coupled polymeric amino columns. New findings about optimization of the derivatisation reaction and chromatographic behaviour. The method was validated using grass samples spiked at the level of 1 mg/kg and gave a detection limit of 0.3 mg/kg for glyphosate and a recovery of 82.4% with RSD of 10.3%. |
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| Keywords: | FMOC-C1 |
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