| 猪圆环病毒诊断方法研究进展 |
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| 引用本文： | 应小飞,杨宗照,应祯灏.猪圆环病毒诊断方法研究进展[J].动物医学进展,2004,25(5):30-32. |
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| 作者姓名： | 应小飞 杨宗照 应祯灏 |
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| 作者单位： | 浙江省杭州市畜牧兽医总站,浙江,杭州,310020;浙江省杭州市畜牧兽医总站,浙江,杭州,310020;浙江大学动物医学研究所,浙江,杭州,310029 |
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| 基金项目： | 杭州市农业发展基金资助项目 |
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| 摘 要： | 猪圆环病毒分为PCV1和PCV2两型,其中PCV2与多种猪病有关,诊断技术显得非常重要。文章综述了PCV2的病理学、血清学、分子生物学等方面的诊断方法。目前已经建立了用原核表达和真核表达的PCV2产物建立的ELISA以及用PCV2单抗建立的ELISA方法;分子生物学方法有竞争PCR、多重PCR、多重巢式PCR、基于TaqManT探针的荧光定量PCR以及先进行PCR再做RFLP区分PCV1和PCV2等方法;核酸原位杂交方法有原位双杂交等,杂交时间可以缩短为2 h。同时检测PCV2和其它病原(如PRRS)的方法已经建立。常规病理学方法利于直观判断,对于病理变化不明显的病例需要用免疫组织化学确诊。血清学方法可以同时处理许多样品,利于大规模普查。PCR方法敏感,在病猪脏器内检测出的阳性率比较高,可以对个体精确诊断,但操作技能水平要求较高。原位杂交方法可以长久保存样品做回顾性的诊断。
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| 关 键 词： | 猪圆环病毒 诊断方法 |
| 文章编号： | 1007-5038(2004)05-0030-03 |
| 修稿时间： | 2003年12月15 |
The Diagnostic Method Progress of Porcine cirovirus |
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| YING Xiao-fei,YANG Zong-zhao,YING Zhen-hao.The Diagnostic Method Progress of Porcine cirovirus[J].Progress In Veterinary Medicine,2004,25(5):30-32. |
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| Authors: | YING Xiao-fei YANG Zong-zhao YING Zhen-hao |
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| Abstract: | Porcine circovirus(PCV) was recently found, and designated type 1 and type 2. PCV2 has do great harm to pigs, so the diagnostic method is very important. This paper described in detail its diagnostic method by (clinical) pathalogy, serology and molecule biology. The traditional pathalogy method is Hematoxylin and eo sin stain,in situ immunohistochemistry(IH) ; Serology method include ELISA based on protein product from prokaryotic expressing system and eukaryotic expressing system or PCV monoclone antibody. Various molecule biology ways, for example, PCR,nest PCR,quantative PCR, competitive PCR, have been set up. In situ hybridization which can be finished in 8 hr has been established. The way that can detect PCV and other pathogens at the same time was also developed. In general, every method has its advantage, ie, pathalogy way is simple , serology method can do with many samples at the same time. PCR is sensitive and quick. When the sample was archived in formalin for a long time , the IH would be the best solution to examine PCV. |
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| Keywords: | Porcine cirovirus diagnostic method |
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