Vegetative compatibility grouping in Botrytis cinerea using sulphate non-utilizing mutants |
| |
| Authors: | N Korolev Y Elad T Katan |
| |
| Institution: | (1) Department of Plant Pathology and Weed Research, Volcani Center, ARO, Bet Dagan, 50250, Israel |
| |
| Abstract: | Twenty-one strains of Botrytis cinerea isolated from six plant species on ten sites throughout Israel, as well as a strain from France, were tested for vegetative
and mycelial incompatibility, pathogenicity, resistance to the fungicides carbendazim and iprodione, and colony morphology.
Selenate-resistant mutants were isolated from the strains as spontaneous, fast-growing sectors arising from restricted colonies
on medium amended with sodium selenate with a mean frequency of 0.04 sectors/colony; 81% of the sectors were sulphate non-utilizing
(sul) mutants. One hundred and four sul mutants were divided into two complementary groups: resistant (66 mutants) and sensitive to chromate. Based on compatibility
reactions between chromate-resistant and chromate-sensitive sul mutants, 12 strains were compatible only with themselves and were each classified as belonging to different vegetative compatibility
groups (VCGs). Nine strains were each compatible with one to three other strains, and were assembled into three multi-member
VCGs. Mycelial incompatibility between wild-type strains (barrage), in the form of a zone of dark pigmentation or sparse mycelium
with or without dark pigmentation of the agar along the line of confrontation, was observed for 70% of the inter-strain pairings.
There was no correspondence in compatibility between strains revealed by two approaches: strains in different VCGs did not
necessarily produce a barrage. However, self-compatibility was observed both as heterokaryon formation between complementary
sul mutants and as an absence of barrages between mycelia of wild-type strains; wild-type strains belonging to the same VCG did
not exhibit strong barrages, although weak antagonistic reactions were observed. Strains in two multi-member VCGs showed the
same patterns of resistance to carbendazim and iprodione; the third multi-member VCG contained isolates with different patterns
of resistance. Four morphological types were revealed among wild-type strains: conidial (five strains), sclerotial (six strains),
intermediate (ten strains), and mycelial (one strain). On bean leaves, conidial strains were more aggressive than sclerotial
strains. |
| |
| Keywords: | Barrage Botrytis cinerea Carbendazim Iprodione Morphological type Phaseolus vulgaris Pathogenicity Sulphate non-utilizing mutants VCG |
| 本文献已被 SpringerLink 等数据库收录！ |
|
