| 橡胶草染色体制片技术研究 |
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| 引用本文： | 杨玉双,甘霖,覃碧,刘实忠.橡胶草染色体制片技术研究[J].热带农业科学,2017(2):31-34. |
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| 作者姓名： | 杨玉双 甘霖 覃碧 刘实忠 |
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| 作者单位： | 1. 中国热带农业科学院橡胶研究所 海南儋州 571737;2. 中国热带农业科学院广州试验站 广东广州 510140 |
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| 基金项目： | 橡胶研究所省部重点实验室及科学观测实验站开放课题 "新型产胶作物橡胶草优异种质筛选及配套栽培技术研究与示范" (No.RRI-KLOF201604)﹔ 橡胶研究所基本科研业务费专项 "重要产胶替代植物种质资源收集与鉴定"(1630022015005) |
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| 摘 要： | 以橡胶草根尖和嫩叶为材料,对其染色体制片技术的取样时间、预处理试剂、解离方法及解离时间进行比较研究。结果表明:橡胶草根尖和嫩叶取样最佳时间均为上午8:00~10:00;2 mmol/L 8-羟基喹啉溶液室温预处理4 h效果最好;根尖和嫩叶用1 mol/L盐酸于60℃下分别酸解10和12 min效果良好。
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| 关 键 词： | 橡胶草 根尖 嫩叶 染色体制片技术 |
Development of Technique for Chromosome Squashing of Taraxacum kok- saghyz Rodin |
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| Yang Yushuang,Gan Lin,Qin Bi,Liu Shizhong.Development of Technique for Chromosome Squashing of Taraxacum kok- saghyz Rodin[J].Chinese Journal of Tropical Agriculture,2017(2):31-34. |
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| Authors: | Yang Yushuang Gan Lin Qin Bi Liu Shizhong |
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| Abstract: | The root tips and young leaves of Taraxacum kok-saghyz Rodin were used for chromosome squashing to compare the differences in chromosome squashing through sampling time, pretreatment chemical solution, hydrolysing methods and time of chromosome squashing. The results showed that the root tips and young leaves were best sampled at about 8﹕ 00~10﹕ 00 am, pretreated in 2 mmol/L 8-hydroxyquinoline solution at a room temperature for 4 hours and hydrolysed with 1 mol/L HCl at 60℃for 10 and 12 min respectively for chromosome squashing. |
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| Keywords: | Taraxacum kok-saghyz Rodin Root tip Young leaf Chromosome squashing technique |
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