| 巴西橡胶树SnRK1家族基因的克隆与表达分析 |
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| 引用本文： | 王 闯,周斌辉,方永军,唐朝荣.巴西橡胶树SnRK1家族基因的克隆与表达分析[J].热带作物学报,2017,38(5):864-872. |
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| 作者姓名： | 王 闯 周斌辉 方永军 唐朝荣 |
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| 作者单位： | 1. 海南大学热带农林学院,海南海口,570228;2. 中国热带农业科学院橡胶研究所,海南儋州,571737 |
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| 基金项目： | 国家863项目(2013AA102605) |
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| 摘 要： | 蔗糖非酵解型蛋白激酶I(Sn RK1)是植物糖信号途径的关键因子,广泛参与植物生长发育。以蓖麻Sn RK1基因序列为探针,采用同源克隆的方法获得了橡胶树Sn RK1家族2个基因的全长c DNA,分别命名为Hb Sn RK1-1(KX237690.1)和Hb Sn RK1-2(KX237691.1)。2个Hb Sn RK1基因均编码含515个氨基酸且序列高度一致(96.5%)的蛋白质;2个基因均含10个外显子、9个内含子,且外显子大小相近,但基因长度差异明显。在6种橡胶树组织中,2个Hb Sn RK1基因均有表达,但Hb Sn RK1-1在胶乳(产胶细胞乳管的细胞质)中的表达量明显高于Hb Sn RK1-2;在叶片发育不同时期,Hb Sn RK1-1的表达变化不显著,而Hb Sn RK1-2的表达随叶片发育明显下降;在胶乳中,Hb Sn RK1-1的表达显著受乙烯和茉莉酸诱导、受2,4-D抑制,但Hb Sn RK1-2表达受激素处理的影响较小,推测Hb Sn RK1可能参与胶乳再生的激素调控。
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| 关 键 词： | 橡胶树 HbSnRK1基因 克隆 生物信息学 表达分析 |
Cloning and Expression Analysis of SnRK1 Genes in Hevea brasiliensis |
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| WANG Chuang,ZHOU Binhui,FANG Yongjun and TANG Chaorong.Cloning and Expression Analysis of SnRK1 Genes in Hevea brasiliensis[J].Chinese Journal of Tropical Crops,2017,38(5):864-872. |
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| Authors: | WANG Chuang ZHOU Binhui FANG Yongjun and TANG Chaorong |
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| Institution: | Institute of Tropical Agriculture and Forestry, Hainan University;Institute of Tropical Agriculture and Forestry, Hainan University;Rubber Research Institute, Chinese Academy of Tropical Agriculture Sciences;Rubber Research Institute, Chinese Academy of Tropical Agriculture Sciences |
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| Abstract: | In higher plants,sucrose non-fermenting 1-related protein kinase 1 (SnRK1) plays a critical role in sugar signaling in relation to growth and development.Here,the full-length cDNAs of two SnRK1 genes were cloned from rubber tree (H.brasiliensis) by homology cloning using a homolog of castor oil plants as the query sequence.The two SnRK1 genes were named as HbSnRK1-1 (KX237690.1) and HbSnRK1-2 (KX237691.1),respectively.Both HbSnRK1 genes predicted a protein of 515 amino acids,and shared a sequence identity of 96.5％.Both HbSnRK1 genes contained 10 exons and 9 introns,but exhibited a large difference in genomic size.Real-time PCR was conducted to characterize the expression patterns of the two HbSnRK1 genes.HbSnRK1-1 and HbSnRK1-2 expressions were detected in all the six Hevea tissues examined,but in the latex (cytoplasm of rubber-producing laticifers),HbSnRK1-1 was much higher expressed than HbSnRK1-2.During the development of Hevea leaves,HbSnRK1-1 expressions showed little change,whereas HbSnRK1-2 expressions decreased progressively.In the latex,the expression of HbSnRK1-1 was significantly stimulated by the treatments of ethylene and jasmonic acid,but repressed by the treatment of 2,4-D.In comparison,the expression of HbSnRK1-2 was much less affected by all the 7 hormones examined.These results suggest HbSnRK1 was involved in hormone-regulated latex regeneration in H.brasiliensis. |
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| Keywords: | Hevea brasiliensis HbSnRK1 gene cloning bioinformatics expression analysis |
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