| 苦丁茶冬青多糖乙醇分级纯化及其理化性质研究 |
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| 引用本文： | 赵书凡,朱科学,朱红英,贺书珍,谭乐和.苦丁茶冬青多糖乙醇分级纯化及其理化性质研究[J].热带农业科学,2017(4):80-86. |
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| 作者姓名： | 赵书凡 朱科学 朱红英 贺书珍 谭乐和 |
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| 作者单位： | 1. 华中农业大学大学食品科学技术学院 湖北武汉430070;中国热带农业科学院香料饮料研究所 海南万宁571533;2. 中国热带农业科学院香料饮料研究所 海南万宁571533;海南省热带香料饮料作物工程技术研究中心 海南万宁571533;3. 海南省热带香料饮料作物工程技术研究中心 海南万宁571533 |
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| 基金项目： | 中央级基本科研业务费(1630142016008)，海南名牌农产品标准制定项目《苦丁茶》 |
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| 摘 要： | 为了研究苦丁茶冬青多糖醇沉规律及产物理化性质。通过热水浸提和不同浓度乙醇分级沉淀,得到乙醇终浓度为20%、40%、60%和80%的4种苦丁茶冬青多糖组分,即ICP-1、ICP-2、ICP-3和ICP-4。比色法检测各多糖组分总糖、糖醛酸、蛋白质和总酚含量;高效凝胶渗透色谱法进行相对分子质量的测定;紫外和红外光谱扫描考察各组分的光谱性质。结果表明:ICP-1、ICP-2、ICP-3和ICP-4总糖含量分别为19.21%、34.91%、37.70%和30.21%,糖醛酸含量分别为5.04%、44.90%、24.20%和5.84%;凝胶渗透色谱图表明:ICP-2和ICP-3为高纯度均一组分,分子量为440.440与348.279 ku;4种多糖组分在280 nm处均有吸收峰,说明均为糖蛋白质化合物。红外结果显示,各组分均为吡喃糖环构型。以上结果表明,乙醇分级沉淀的方法可用于分级纯化制备组分均一、纯度较高的苦丁茶冬青多糖组分。
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| 关 键 词： | 苦丁茶冬青 多糖 理化性质 光谱性质 |
Ethanol Fractionation and Physico-chemical Properties of Soluble Polysaccharides from Ilex kudingcha C.J.Tseng |
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| ZHAO Shufan,ZHU Kexue,ZHU Hongying,HE Shuzhen,TAN Lehe.Ethanol Fractionation and Physico-chemical Properties of Soluble Polysaccharides from Ilex kudingcha C.J.Tseng[J].Chinese Journal of Tropical Agriculture,2017(4):80-86. |
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| Authors: | ZHAO Shufan ZHU Kexue ZHU Hongying HE Shuzhen TAN Lehe |
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| Abstract: | Ilex kudingcha C.J.Tseng was extracted with hot water and then fractionated with ethanol to produce different concentrations of water-soluble polysaccharides,and the physico-chemical properties of these polysaccharides was analyzed.The polysaccharides were fractionated and purified into fractions with concentrations of 20％,40％,60％ and 80％,and were named ICP-1,ICP-2,ICP-3 and ICP-4,respectively.The polysaccharides were detected by using colometrics in terms of total sugar,protein,alduronic acid and total phenol;their molecular weight (Mw) was determined by using high performance gel permeation chromatography,and their spectroscopic properties by using UV and IR spectra analysis.The polysaccharides fractions ICP-1,ICP-2,ICP-3 and ICP-4 were detected to contain 19.21％,34.91％,37.70％ and 30.21％ of total sugar,respectively,and their alduronic acid contents were 5.04％,44.90％,24.20％ and 5.84％,respectively.The gel permeation chromatograph showed that ICP-2 and ICP-3 were of high and uniform purity,their Mw being estimated about 440.440 and 348.279 ku.The UV spectra showed that these four fractions all had a characteristic absorption peak at near 280 nm.The IR spectrum indicated that all the fractions had a structure of pyranose ring.These results showed that this extraction and fractionation method can be used to produce uniformly pure water-soluble polysaccharides with higher concentrations from I.kudingcha C.L Tseng. |
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| Keywords: | Ilex kudingcha C J Tseng polysaccharides physicochemical properties spectroscopic properties |
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