| 大根唇柱苣苔的组培快繁技术 |
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| 引用本文： | 林茂,李进华,唐庆,王华新,廖美兰,龙定建.大根唇柱苣苔的组培快繁技术[J].热带作物学报,2017(3):508-512. |
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| 作者姓名： | 林茂 李进华 唐庆 王华新 廖美兰 龙定建 |
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| 作者单位： | 广西壮族自治区林业科学研究院,广西南宁,530002 |
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| 基金项目： | 广西林业科技项目(桂林科字2013第5号)，广西自然科学基金项目(2014GXNSFBA118091)，国家林业局部门预算广西项目(桂林护预2015007)，广西壮族自治区林业科学研究院基本科研业务费项目(林科201405号) |
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| 摘 要： | 以大根唇柱苣苔叶片为外植体,以MS为基本培养基,附加不同种类和浓度的激素进行组培快繁技术研究。结果表明:适宜叶片不定芽诱导的培养基是MS+6-BA 1.00 mg/L+NAA 0.05 mg/L,继代增殖培养的适宜培养基为MS+6-BA 1.50~3.00 mg/L+NAA 0.10 mg/L,适宜的生根培养基为1/2MS+IBA 0.20~0.30 mg/L,适宜的基质是泥炭∶珍珠岩=2∶1、泥炭∶细河沙=2∶1、泥炭∶园土=2∶1。
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| 关 键 词： | 大根唇柱苣苔 组培 快繁 不定芽 |
Tissue Culture and Rapid Regeneration of Chirita macrorhiza |
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| LIN Mao,LI Jinhua,TANG Qing,WANG Huaxin,LIAO Meilan,LONG Dingjian.Tissue Culture and Rapid Regeneration of Chirita macrorhiza[J].Chinese Journal of Tropical Crops,2017(3):508-512. |
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| Authors: | LIN Mao LI Jinhua TANG Qing WANG Huaxin LIAO Meilan LONG Dingjian |
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| Abstract: | The leaf of Chirita macrorhiza was used as the explants and cultured on MS medium supplemented with different concentrations of plant hormones.The results showed that:the adventitious bud induction culture medium was MS+6-BA 1.00 mg/L+NAA 0.05 mg/L.The shoots proliferation medium was MS+6-BA 1.50-3.00 mg/L+NAA 0.10 mg/L.The root induction medium was 1/2MS+IBA 0.20-0.30 mg/L.The suitable ratio of transplanting medium for peat ∶ perlite was 2 ∶ 1,peat ∶ fine sand is 2 ∶ 1,and peat ∶ soil was 2 ∶ 1. |
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| Keywords: | Chirita macrorhiza tissue culture rapid regeneration adventitious bud |
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