| 高效液相色谱法定性定量测定油梨果实中的可溶性糖成分 |
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| 引用本文： | 葛 宇,曹剑秋,司雄元,胡福初,李 章,臧小平,马蔚红.高效液相色谱法定性定量测定油梨果实中的可溶性糖成分[J].热带农业科学,2017,37(7):89-94. |
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| 作者姓名： | 葛 宇 曹剑秋 司雄元 胡福初 李 章 臧小平 马蔚红 |
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| 作者单位： | 1. 中国热带农业科学院海口实验站 海南海口 570102;2. 海南大学园艺园林学院 海南海口 570228;3. 安徽农业大学生物技术中心 安徽合肥 230036;4. 海南省农业科学院热带果树研究所/海南省热带果树生物学重点实验室 海南海口 571100 |
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| 基金项目： | 海南省自然科学基金 "基于脂肪酸指纹图谱的油梨品质相关研究"(20163096),中国热带农业科学院基本科研业务费专项资金 "贵州兴义油梨品种筛选及高效生产技术研发与集成示范"(2017125141),农业部物种资源保护(农作物)项目 "热带珍稀野生果树资源调查、 收集与评价"(1251416305010) |
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| 摘 要： | 分析油梨果实可溶性糖成分及含量,为以后深入分析、利用其成分提供一定理论基础。对所采集的3份油梨,应用高效液相色谱仪测定其可溶性糖组分及含量。结果显示,3份油梨果肉中的可溶性总糖含量为(13.19±0.32)~(25.94±0.98)(mg/g FW),其可溶性糖由甘露庚酮糖、甘露庚糖醇、果糖及葡萄糖等4个成分组成,以甘露庚酮糖为主;可溶性总糖、甘露庚酮糖、甘露庚糖醇、果糖及葡萄糖含量在3份油梨果肉中差异均显著。3份油梨种子中的可溶性总糖含量为(20.47±0.52)~(29.41±0.60)(mg/g FW),其可溶性糖同样由甘露庚酮糖、甘露庚糖醇、果糖及葡萄糖等4个成分组成,以甘露庚糖醇为主;可溶性总糖、甘露庚酮糖、甘露庚糖醇、果糖及葡萄糖含量在3份油梨种子中差异均显著。3份油梨果肉和种子之间可溶性总糖和各可溶性糖成分平均含量差距大小依次为:甘露庚糖醇果糖甘露庚酮糖葡萄糖可溶性总糖。
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| 关 键 词： | 油梨 果肉和种子 可溶性糖成分 高效液相色谱 |
Identification and Quantitation of Soluble Sugar Components of Avocado Fruit by High Performance Liquid Chromatography |
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| GE Yu,CAO Jianqiu,SI Xiongyuan,HU Fuchu,LI Zhang,ZANG Xiaoping,MA Weihong.Identification and Quantitation of Soluble Sugar Components of Avocado Fruit by High Performance Liquid Chromatography[J].Chinese Journal of Tropical Agriculture,2017,37(7):89-94. |
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| Authors: | GE Yu CAO Jianqiu SI Xiongyuan HU Fuchu LI Zhang ZANG Xiaoping MA Weihong |
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| Institution: | 1 Haikou Experimental Station, CATAS, Haikou, Hainan 570102;2 College of Horticulture and Landscape Architecture, Hainan University, Haikou, Hainan 570228;3 Biotechnology Center, Anhui Agricultural University, Hefei, Anhui 230036;4 Institute of Tropical Fruit Tree, Hainan Academy of Agricultural Sciences / Hainan Key Laboratory of Tropical Fruit Tree Biology, Haikou, Hainan 571100;2 College of Horticulture and Landscape Architecture, Hainan University, Haikou, Hainan 570228;1 Haikou Experimental Station, CATAS, Haikou, Hainan 570102;1 Haikou Experimental Station, CATAS, Haikou, Hainan 570102 |
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| Abstract: | Avocado fruits (Persea americana Mill.) were collected from 3 accessions of avocado germplasm and their soluble sugar components and contents were determined. The results showed that the contents of total soluble sugar in the pulps of the avocado fruits of the 3 avocado accessions ranged from (13.19 ± 0.32) to (25.94 ± 0.98) mg/g FW. The soluble sugar components were composed of mannoheptulose,perseitol, fructose and glucose, and dominated by mannoheptulose. There were significant differences in the contents of total soluble sugar, mannoheptulose, perseitol, fructose and glucose among the avocado fruits of 3 avocado accessions. The total soluble sugar contents in the seeds of the 3 avocado accessions were at the range of between (20.47 ± 0.52) and (29.41 ± 0.60)mg/g FW. The soluble sugar component also included perseitol, mannoheptulose, fructose and glucose, dominated by perseitol. There were great differences between the contents of total soluble sugar, mannoheptulose, perseitol, fructose and glucose of the seeds of 3 avacado germplasms. The average contents of total soluble sugar and other soluble sugar components in the fruit pulp and seeds of 3 avocado accessions was in the order of perseitol >fructose >mannoheptulose>glucose>total soluble sugar. |
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| Keywords: | Persea americana Mill pulp and seed soluble sugar components high performance liquid |
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