| 巴西橡胶树生长素响应HbJAR1基因克隆与表达分析 |
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| 引用本文： | 李晓娜,肖厚贞,万三连,张冬,张宇.巴西橡胶树生长素响应HbJAR1基因克隆与表达分析[J].热带作物学报,2017(8):1478-1484. |
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| 作者姓名： | 李晓娜 肖厚贞 万三连 张冬 张宇 |
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| 作者单位： | 1. 中国热带农业科学院科技信息研究所,海南海口,571101;2. 海南大学材料与化工学院,海南海口,570228;3. 三亚市现代农业检验检测预警防控中心,海南三亚,572000;4. 海南省热带生物资源可持续利用重点实验室海南大学热带农林学院 海南海口 570228 |
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| 基金项目： | 国家自然科学基金(31660204、 31660187)，中国热带农业科学院基本科研业务费专项资金(1630072016005) |
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| 摘 要： | JAR1基因是植物生长素响应基因,具有维持植物体内生长素浓度动态平衡和增强植物抗逆性的功能。为了阐明巴西橡胶树中JAR1基因的结构与功能,本研究利用Q-PCR技术在橡胶树CATAS7-33-97叶片中扩增了JAR1基因,其推导氨基酸含有JAR1蛋白特征性的GH3 superfamily结构域,无信号肽,无跨膜区域,定位在细胞质中,命名为HbJAR1。表达分析结果表明:该基因在橡胶树叶片中的表达量受光照强度、机械伤害和白粉菌侵染显著上调,吲哚乙酸(IAA)、茉莉酸(JA)、乙烯利(ET)、脱落酸(ABA)和赤霉素(GA3)对HbJAR1在橡胶树中的表达也起到不同程度的上调作用。表明HbJAR1基因属于植物JAR1家族,与橡胶树对光照、伤害等逆境响应有关。
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| 关 键 词： | 巴西橡胶树 HbJAR1 基因克隆 生物信息学 表达分析 |
Gene Cloning and Expression Analysis of Auxin Responsive HbJAR1 in Rubber Tree |
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| LI Xiaona,XIAO Houzhen,WAN Sanlian,ZHANG Dong,ZHANG Yu.Gene Cloning and Expression Analysis of Auxin Responsive HbJAR1 in Rubber Tree[J].Chinese Journal of Tropical Crops,2017(8):1478-1484. |
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| Authors: | LI Xiaona XIAO Houzhen WAN Sanlian ZHANG Dong ZHANG Yu |
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| Abstract: | JA R1, a auxin response gene, maintains dynamic balance of auxin concentration in plant, strengthens plant tolerance ability stress. To clarify the gene structure and function of JAR1 in rubber tree (Hevea brasiliensis Müll. Arg.), HbJAR1 gene was cloned in the leaves of rubber tree CATAS7-33-97 by RT-PCR. The deduced amino acid of HbJAR1 protein has special GH3 superfamily domain structure, no signal peptide, no transmembrane region, located in the cytoplasm. The gene expression of HbJAR1 in the leaves of rubber tree was significantly affected by light intensity, mechanical wounding and powdery mildew infection. IAA, GA3, ET, JA and ABA treatments also induced HbJAR1 expression at different levels. The results suggested that HbJAR1 is a member of plant JAR1 gene family, involved in stress response such as light and wounding signal in rubber tree, etc. |
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| Keywords: | Hevea brasiliensis Müll Arg HbJAR1 gene clone bioinformatics expression analysis |
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