| 齿兰环斑病毒外壳蛋白基因的表达及其表达产物抗血清的制备 |
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| 引用本文： | 潘俊松,刘志昕.齿兰环斑病毒外壳蛋白基因的表达及其表达产物抗血清的制备[J].热带作物学报,1999,20(2):62-66. |
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| 作者姓名： | 潘俊松 刘志昕 |
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| 作者单位： | 中国热带农业科学院热带作物生物技术国家重点实验室!海口，571101 |
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| 摘 要： | 用pET－21d构建齿兰环斑病毒（ORSV）外壳蛋白基因大肠杆菌表达载体pEO。经SDS－PAGE和Westernblotting分析，含pEO的大肠杆菌正确表达了ORSV外壳蛋白基因。此表达产物为融合蛋白。用表达产物制备抗血清，并应用于间接酶联法检测ORSV，具有较高的灵敏度（10ng／mLORSV）和特异性。
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| 关 键 词： | 齿兰环斑病毒 外壳蛋白基因 抗血清 |
Expression in Escherichia coli of Odontoglossum Ringspot Virus Coat Protein Gene and Preparation of Antiserum of the Product |
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| Pan Junsong, Liu Zhixin, Kang Youfa, Zheng Xueqin.Expression in Escherichia coli of Odontoglossum Ringspot Virus Coat Protein Gene and Preparation of Antiserum of the Product[J].Chinese Journal of Tropical Crops,1999,20(2):62-66. |
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| Authors: | Pan Junsong Liu Zhixin Kang Youfa Zheng Xueqin |
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| Abstract: | The expression vector pEO in Escherichia coli of the ondontoglossum ringspot virus (ORSV) coat protein gene was constructed by using pET-21d. The ORSV coat protein gene was expressed correctly in E.coli of pEO when tested by SDS-PAGE and Western blotting analysis. The antiserum of the expressed product was prepared by rabbit immunization and was found highly sensitive (10ng/mL ORSV) and specific when used to detect ORSV in indirect-ELISA. |
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| Keywords: | Odontoglossum ringspot virus coat protein antiserum |
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