传染性法氏囊病病毒BC6/85株全基因组克隆及其序列分析 |
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引用本文: | 于雷,李慧姣.传染性法氏囊病病毒BC6/85株全基因组克隆及其序列分析[J].中国兽药杂志,2013,47(12):17-21. |
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作者姓名: | 于雷 李慧姣 |
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作者单位: | 中国兽医药品监察所,中国兽医药品监察所 |
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摘 要: | 以传染性法氏囊病病毒(IBDV)BC6/85株基因组RNA为模板,采用RT—PCR方法扩增并克隆了IBDVBC6/85株基因组全长eDNA。序列测定结果表明:A节段全长共3260个核苷酸,与IM株的同源性最高为97.4%,与其他血清I型毒株的核苷酸同源性介于91.2%~97.1%;B节段有2827个核苷酸,与IM株同源性最高为98.6%,与其他毒株的核苷酸同源性为88.7%~98.6%。通过对编码的氨基酸序列进行分析,发现BC6/85株有21个特有氨基酸,其中15个位于多聚蛋白VP2—4—3。系统进化树分析表明,BC6/85株与经典毒株、弱毒株和变异株的关系较近,而与超强毒株相对较远。
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关 键 词: | 传染性法氏囊病病毒 全基因组 序列分析 |
收稿时间: | 2013/6/28 0:00:00 |
修稿时间: | 2013/7/23 0:00:00 |
Sequence Analysis of the Complete Genome of IBDV BC6/85 |
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Institution: | China Institute of Veterinary Drug Control, |
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Abstract: | The full-length of double segments of the infectious bursal disease virus (strain BC6/85) was amplified by RT-PCR and sequenced. The result showed that segment A contains 3260bp,compared with other IBDV strains,BC6/85 segment A shared 91.2%~97.4% homology at nucleotide level,segment B contains 2827bp,which shared 88.7%~98.6% homology. The analysis of deduced amino acid sequences indicated that IBDV BC6/85 strain has 21 specific residues. Phylogenetic tree analysis indicated BC6/85 is more closely related to the attenuated, classical and variant IBDV than those of very virulent IBDV strains. |
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Keywords: | infectious bursal disease virus complete genome sequence analysis |
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