| 簇毛麦HMW-GS基因的位点特异PCR(AS-PCR)标记及其多态性 |
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| 引用本文： | 刘守斌,梁荣奇,尤明山,李保云,刘广田.簇毛麦HMW-GS基因的位点特异PCR(AS-PCR)标记及其多态性[J].华北农学报,2011,26(1):1-5. |
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| 作者姓名： | 刘守斌 梁荣奇 尤明山 李保云 刘广田 |
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| 作者单位： | 中国农业大学,农学与生物技术学院,植物遗传育种系,农业生物技术国家重点实验室,北京市作物遗传改良重点实验室,农业部作物基因组与遗传改良重点实验室,北京,100193 |
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| 基金项目： | 国家自然科学基金项目(39930110); 北京市自然科学基金项目(6990001) |
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| 摘 要： | 簇毛麦含有丰富的HMW-GS基因变异.本研究旨在建立簇毛麦HMW-GS基因的位点特异PCR(AS-PCR)标记,并对18个居群簇毛麦HMW-GS基因遗传多样性进行系统评价,为开发和利用簇毛麦HMW-GS改良普通小麦品质奠定基础.根据先前克隆的3个簇毛麦HMW-GS基因序列(1Va、1Vb和1Vc)和已发表的普通小麦HM...
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| 关 键 词： | 簇毛麦 高分子量谷蛋白亚基(HMW-GS) 位点特异PCR(AS-PCR)标记 多态性 |
The Allelic Specific PCR Marker and Diversity of HMW-GS Genes in Dasypyum villosum |
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| LIU Shou-bin,LIANG Rong-qi,YOU Ming-shan,LI Bao-yun,LIU Guang-tian.The Allelic Specific PCR Marker and Diversity of HMW-GS Genes in Dasypyum villosum[J].Acta Agriculturae Boreali-Sinica,2011,26(1):1-5. |
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| Authors: | LIU Shou-bin LIANG Rong-qi YOU Ming-shan LI Bao-yun LIU Guang-tian |
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| Institution: | LIU Shou-bin,LIANG Rong-qi,YOU Ming-shan,LI Bao-yun,LIU Guang-tian(State Key Laboratory for Agrobiotechnology,Beijing Key Laboratory of Crop Genetic Improvement,Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100094,China) |
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| Abstract: | D.villosum is a rich reservoir for genic diversity of High-Molecular-Weight glutenin subunit(HMW-GS).In this paper,one Allelic Specific PCR(AS-PCR)marker for the amplification of the genes coding HMW-GS of D.villosum was developed on the basis of the sequences of 1Va,1Vb,1Vc and common wheat HMW glutenin genes.PCR analysis on HMW glutenin genes of CS and its addition lines showed the specialty of this AS-PCR marker,while analysis on HMW-GS genes of six accessions of D.villosum showed a high degree of length... |
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| Keywords: | Dasypyum villosum HMW-GS Allelic specific PCR(AS-PCR)marker Diversity |
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