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| 用基因枪法将Bt杀虫基因导入水稻愈伤组织的研究 | |
| 引用本文： | 贝丽霞,王守德,史芝文,徐仲,郭三堆.用基因枪法将Bt杀虫基因导入水稻愈伤组织的研究[J].东北农业大学学报,2001,32(1):90-94. |
| 作者姓名： | 贝丽霞 王守德 史芝文 徐仲 郭三堆 |
| 作者单位： | 1. 黑龙江八一农量大学植物科技学院 2. 东北农业大学生物工程系 3. 中国农业科学院生物技术研究中心 |
| 摘 要： | 从水稻成熟胚诱导出的愈伤组织经继代培养后可以产生大量的胚性愈伤组织。本研究采用基因枪法转化上述胚性愈伤组织 ,在附加羧苄青霉素 (5 0 0 μg· m L-1)和卡那霉素 (5 0 μg·m L-1)的选择培养基上进行抗性愈伤组织的筛选 ,在附加羧苄青霉素 (5 0 0 μg· m L-1)和卡那霉素 (2 5 μg· m L-1)的分化培养基上诱导抗性芽。GUS酶活性检测和对基因组 DNA作 PCR检测结果均为阳性 ,证明外源目的基因 (Bt)已在寒地水稻抗性愈伤组织中整合并表达。抗性再生植株的 GUS酶活性及 PCR检测正在进行中 |
| 关 键 词： | 水稻 基因枪 Bt基因 遗传转化 |
| 文章编号： | 1005-9369(2001)01-00 |
| 修稿时间： | 2000年3月15日 |
Regenerative rice plants obtained by microprojectile bombardent of embrogenic calli |
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| BEI Li-xia,WANG Sho-de,SHI Zhi-wen,XU Zhong,GUO Shan-dui.Regenerative rice plants obtained by microprojectile bombardent of embrogenic calli[J].Journal of Northeast Agricultural University,2001,32(1):90-94. | |
| Authors: | BEI Li-xia WANG Sho-de SHI Zhi-wen XU Zhong GUO Shan-dui |
| Abstract: | Many embryogenic calli were produced through subculture by calli induced from mature embryo of rice. The above-mentioned calli were transformed by using Microprojectile bombardment, and resistant calli were selected on selective media containing Cb (500 μg ·mL-1) and Km (50μg · mL-1). The resistant shoots were induced on differentiation media containing Cb (500 μg · mL-1) and Km (25 tμg · mL-1). Finally, regenerative plantlets were obtained, the result is positive by Gus enzyme activity test and PCR test, Showing that foreign gene (Bt) had been integrated into and expressed in Oryza sativa L. resistand calli was testified.Gus enzyme activity test and PCR test of resistant plantlets are in progress. |
| Keywords: | Oryza sativa L Microprojectile bombardment Bacillus thuringiensis gene Genetic transformation |
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