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| 生防因子螺旋毛壳ND35的细胞壁降解酶与重寄生作用 | |
| 引用本文： | 高克祥,刘晓光,Dana Friesem,Leonid Chernin,时呈奎.生防因子螺旋毛壳ND35的细胞壁降解酶与重寄生作用[J].林业科学,2005,41(1):205-210. |
| 作者姓名： | 高克祥 刘晓光 Dana Friesem Leonid Chernin 时呈奎 |
| 作者单位： | 1. 西北农林科技大学植物保护学院,杨凌,712100 2. 山东农业大学植物保护学院,泰安,271018 3. 希伯莱大学农业、食品与环境科学院植病和微生物系及农业生物技术中心,76100以色列 |
| 基金项目： | ThisstudywasFundedbytheNationalNaturalScienceFoundationofChina (NSFC) (3 0 10 0 14 3 ) . |
| 摘 要： | 1 IntroductionSomespeciesofthesaprophyticascomycetegeneraChaetomiumKunzeexFr .arepotentialantagonistsofseveralplantpathogenicfungi ,forexampleRhizoctoniasolaniandPythiumultimumwhichcauseseedlingdamping -offandVenturiainaequaliswhichcausestheapplescabChae… |
| 关 键 词： | 螺旋毛壳ND35 细胞壁降解酶 活性检测 重寄生 扫描电镜 |
Cell Wall Degrading Enzymes Involved in Mycoparasitism of the Biocontrol Agent Chaetomium spirale ND35 |
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| Gao Kexiang,Liu Xiaoguang,Dana Friesem,Leonid Chernin,Shi Chengkui.Cell Wall Degrading Enzymes Involved in Mycoparasitism of the Biocontrol Agent Chaetomium spirale ND35[J].Scientia Silvae Sinicae,2005,41(1):205-210. | |
| Authors: | Gao Kexiang Liu Xiaoguang Dana Friesem Leonid Chernin Shi Chengkui |
| Institution: | Gao Kexiang1 Liu Xiaoguang2 Dana Friesem3 Leonid Chernin3 Shi Chengkui2 |
| Abstract: | Some Chaetomium spp. Are capable of antagonizing several plant pathogenic fungi through production of antibiotics and mycoparasitism. Secretion of lytic enzymes, mainly including glucanases and chitinases, is considered the most important step in the mycoparasitic process. In this study, an about 110kDa exo - β - 1,3 - glucanase from C. Spirale ND35 was detected both in culture filtrate and directly on PAGE and IEF gels, as well as chitinases, although protease was not detectable on Litmus milk agar plates. Coiling and penetrating the hyphae of host fungus Valsa mali were observed by scanning electron microscope (SEM), which may be related to the synergistic interaction between β - 1,3 - glucanase and chitinases. Β - 1,3 - glucanase activity of C. Spirale ND35 varied considerably when C. Spirale ND35 was grown in different carbon sources during various incubation time, and might be subjected to both induction by substrate and catabolite repression. |
| Keywords: | Chaetomium spirale ND35 cell wall degrading enzymes assays of enzyme activities mycoparasitism scanning electron microscope |
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