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| 苏云金杆菌HBF-1菌株在土壤中毒蛋白含量的ELISA检测 | |
| 引用本文： | 吴瑞华,李国勋,王容燕,王金耀,冯书亮.苏云金杆菌HBF-1菌株在土壤中毒蛋白含量的ELISA检测[J].中国生物防治,2007,23(3):263-268. |
| 作者姓名： | 吴瑞华 李国勋 王容燕 王金耀 冯书亮 |
| 作者单位： | 1. 河北农业大学植物保护学院,保定,071001;河北省农林科学院植保所,保定,071000 2. 莱阳农学院,青岛,266109 3. 河北省农林科学院植保所,保定,071000 |
| 基金项目： | 国家高技术研究发展计划(863计划);河北省自然科学基金;河北省农科院青年基金 |
| 摘 要： | 以苏云金芽孢杆菌HBF-1菌株中分离纯化的高特异性杀虫蛋白作为抗原，免疫新西兰白兔制备出多克隆抗体，其效价可达到16000。应用制备的特异性抗体建立了间接酶联免疫吸附测定法（ELISA），检测不同土壤样品中Bt毒蛋白含量。初步结果表明，该方法能够检测土壤中Bt毒蛋白的含量，因土壤理化特性及组成成分的不同，检测到的毒蛋白含量也略有不同，河沙中的蛋白检测量最低，只有12．53％；另一种沙壤混合物中检测量达到76.16％。 |
| 关 键 词： | HBF-1菌株 毒蛋白 多克隆抗体 间接ELISA 土壤 |
| 文章编号： | 1005-9261（2007）03-0263-06 |
| 修稿时间： | 2006-09-26 |
Detection of Bacillus thuringiensis Strain HBF-1 Protoxin in Soil by Enzyme-linked Immunosorbent Assay |
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| WU Rui-hua,LI Guo-xun,WANG Rong-yan,WANG Jin-yao,FENG Shu-liang.Detection of Bacillus thuringiensis Strain HBF-1 Protoxin in Soil by Enzyme-linked Immunosorbent Assay[J].Chinese Journal of Biological Control,2007,23(3):263-268. | |
| Authors: | WU Rui-hua LI Guo-xun WANG Rong-yan WANG Jin-yao FENG Shu-liang |
| Institution: | College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China |
| Abstract: | With the SDS-PAGE preparation electrophoresis, high quality insecticidal protein was purified from a unique Bacillus thuringiensis serovar japonensis strain HBF-1, which is highly toxic to larvae of Anomala exoleta and A. corpulentathe. The protein was used as antigen and high immunity antiserum was got after several times of immuning to rabbits with titer reaching 1/16000. With the specific antiserum, a method for detecting the presence of the protoxin was developed. The Bt protoxin in different soil was determined by indirect ELISA. Results indicated that detection of the toxin was affected by soil texture. Detected rate in sandy soil was 12.53% only, and 76.16% in sandy loam soil. |
| Keywords: | Bacillus thuringiensis strain HBF-1 insectical protein polyclonal ID-ELISA soil |
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