鹿药组织培养的研究 |
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引用本文: | 安晓云,慈嘉,申琼.鹿药组织培养的研究[J].中国园艺文摘,2010,26(10):13-15. |
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作者姓名: | 安晓云 慈嘉 申琼 |
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作者单位: | 1. 辽宁师范大学生命科学学院,辽宁,大连,116029 2. 大连周水子机场飞保部,辽宁,大连,116033 |
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摘 要: | 为保护鹿药这种野生植物资源,以生长旺盛的鹿药根茎为材料,进行愈伤组织诱导、愈伤组织分化、试管苗的生根、扦插和移植的研究,成功地建立起壳药根茎的再生体系。结果证明:MS+ZT0.2mg/L+CH20mg/L+2,4-D1.5mg/L(或2.0mg/L)是鹿药根茎愈伤组织诱导培养的理想培养基;MS+AgNO3 0.2mg/L+BA0.2mg/L+KT0.3mg/L+NAA0.1mg/L是鹿药愈伤组织分化培养的理想培养基;1/2MS+BA0.4mg/L+NAA0.1mg/L是鹿药愈伤组织分化不定芽继代培养和增殖培养的理想培养基;1/3MS+NAA0.1mg/L+IAA0.4mg/L是鹿药生根培养和生根继代培养的理想培养基;移植到山坡上试管苗生长旺盛。
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关 键 词: | 鹿药 组织培养 培养基 |
Study of Tissue Culture of Smilacina laponica |
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Authors: | AN Xiao-Yun CI Jia SHEN Qiong |
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Institution: | AN Xiao-Yun CI Jia SHEN Qiong |
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Abstract: | For the protection of wild resources, the exptants from strong growth Smilacina laponica stem segment were cultured to in- vestigate the effects of different external hormones on induction and differentiation of callus, formation of adventitious roots, cutting and transplantation. The asexual line of Smilacina laponica was MS+ZT established successfully. The results show that the ideal medium for induction and differentiation of callus were MS+ZT0.2mg/L+CH20mg/L+2, 4-D1.5mg/L(or 2.0mg/L)and MS+AgNO30.2mg/L+ BA0.2mg/L+KT0.3mg/L+NAA0.1mg/L respectively, the ideal differentiation and multiplication medium for adventitious buds was 1/ 2MS+BA0.4mg/L+NAA0.1mg/L, the ideal medium for rooting was 1/3MS+NAA0.1mg/L+IAA0.4mg/L. The tube seedlings transplanted to the hill grew orderly and flourishly. |
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Keywords: | Smilacina laponica culture of tissue medium |
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