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非淀粉多糖酶对牙鲆生长和饲料利用率的影响
作者姓名:陈京华
作者单位:青岛农业大学动物科技学院,山东青岛,266109 ?
基金项目:国家自然科学基金,青岛农业大学高层次人才启动基金?
摘    要:以初始平均体重(2.04±0.02)g的牙鲆(Paralichthys olivaceus )为实验对象,进行为期70天的摄食生长实验,研究不同添加方式的非淀粉多糖酶对牙鲆生长和饲料利用率的影响.在5000.0 g豆粕中添加25.0 g非淀粉多糖酶,然后用产朊假丝酵母(Candida utilis)进行发酵预处理,得到非淀粉多糖酶预处理豆粕.共制作4种等氮等能饲料,其中以全鱼粉饲料为对照饲料,用豆柏蛋白替代45%的鱼粉蛋白配制成豆粕组饲料;在豆粕组饲料中添加0.2%非淀粉多糖酶配制成非淀粉多糖酶组饲料:用非淀粉多糖酶预处理豆粕蛋白替代45%的鱼粉蛋白配制成非淀粉多糖酶预处理豆粕组饲料.结果表明,用豆粕蛋白替代饲料中45%的鱼粉蛋白,若不添加非淀粉多糖酶则显著降低牙鲆的特定生长率(P<0.01)、饲料效率、蛋白质效率和氮贮积率(P<0.05);在含豆粕饲料中添加0.2%非淀粉多糖酶显著提高牙鲆的特定生长率(P<0.01)、饲料效率、蛋白质效率、氮和磷贮积率,显著降低氮和磷排放率(P<0.05);在饲料中添加非淀粉多糖酶预处理豆粕显著提高牙鲆的特定生长率(P<0.01),但对饲料效率、蛋白质效率无显著的影响(P>0.05).

关 键 词:牙鲆  豆粕  非淀粉多糖酶  生长  饲料利用率

Effects of Non-starch Polysaccharide Enzyme on Growth and Feed Utilization of Japanese flounder (Paralichthys olivaceus)
Authors:Chen Jinghua
Institution:(College of Animal Science and Technology, Qingdao Agricultural University, Qingdao Shandong 266109)
Abstract:Abstract: A study was conducted to evaluate the effects of non-starch polysaccharide (NSP) enzyme on growth and feed utilization of Japanese flounder (Paralichthys olivaceus). The NSP-enzyme pretreated soybean meal (SBM) was obtained by fermentation with Candida utilis. Four isonitrogenous and isocaloric diets were formulated. The control diet contained 68.0% fish meal (FM) as the main protein source, whereas 45% of FM protein was replaced by SBM protein in the SBM diets with 0 and 0.2% NSP-enzyme. The NSP-enzyme pretreated SBM diet was formulated in which NSP-enzyme pretreated SBM protein replaced 45% of FM protein. At the end of the feeding trial, the specific growth rate (SGR), feed efficiency ratio (FER), protein efficiency ratio (PER) and nitrogen retention of fish fed the SBM diet without NSP-enzyme were significantly lower than those in control (P<0.05). Comparing with the SBM diet without NSP-enzyme, the fish fed the SBM diet supplemented NSP-enzyme showed significantly higher SGR (P<0.01), FER, PER, nitrogen and phosphorus retention (P<0.05), and significantly lower nitrogen and phosphorus load (P<0.05). The SGR of fish fed NSP-enzyme pretreated SBM diet was significantly higher than that of the SBM diet without NSP-enzyme (P<0.01), however FER and PER were not significantly different from other treatments (P>0.05).
Keywords:Key words: Japanese flounder Paralichthys olivaceuszz  soybean mealzz  NSP-enzymezz  growthzz  feed utilizationzz
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