| 无病毒百合组培种球快速繁殖体系的建立立 |
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| 作者姓名： | 徐品三 刘华夏 |
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| 作者单位： | 大连理工大学环境与生命学院,大连,116023
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| 基金项目： | 国家自然科学基金，大连市科技局计划项目? |
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| 摘 要： | 以铁炮百合(Lilium Longiflorum)感病种球为材料,对热处理后的种球进行了培养基附加物和移栽基质的优化,建立了无病毒组培种球快速繁殖体系.结果表明:以MS为基本培养基,当附加植物激素N从和KT时能促进子球再生,再生率达86%以上,在0.2mg,LN从和0.4mg/LKT的培养基中平均再生子球数显著增多;附加5 mg/L多效唑培养基能促进子球增大,3 mg/L多效唑有利于子球再分化;活性炭对子球增大无明显影响,但适当浓度的活性炭能提高培养子球的品质;高浓度蔗糖能促进子球增大,但对子球再分化有抑制影响.蛭石:土、珍珠岩以及珍珠岩:土作为基质时,均能达到较高的成活率和株高,但在珍珠岩:土为3:1作为基质移栽组培球,其成活率和生长状况最佳.37℃热处理随着处理天数的增加,处理后培养鳞片的成活率和子球再生率显著下降,但处理20天子球再生率只能达40%左右,但LSV检出率明显低于未处理的种球,脱毒率达95%以上.
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| 关 键 词： | 百合 热处理 病毒检测 子球快繁 户外移栽 |
Establishment of In Vitro Rapid Propagation System on Virus-free Bulblets of Lilium spp. |
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| Authors: | Xu Pinsan Liu Huaxia |
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| Abstract: | Virus-free in vitro bulblets rapid propagation system was established by optimizing the medium accretion and transplantation matrix of the bulbs after heat treatment using Lilium Longiflorum bulbs with virus as experimental material. The results showed that the regeneration rate was up to more than 86% when added MS with phytohormone NAA and KT which can promote the bulblets formation. The best bulblets formation was observed in the medium with 0.4mg?L-1 KT and 0.2mg?L-1 NAA. Optimum bulblet enlargement was observed in medium with Paclobutrazol 5mg/L, while Paclobutrazol in concentration 3mg?L-1 was propitious to bulblet formation. The bulblets enlargement can not be affected significantly by AC, but the quality of bulblets can be enhanced in the medium with appropriate concentration of AC. Medium with higher concentration sucrose can promote bulblet enlargement but inhibit bulblet formation. The best matrix for the highest surviving rate and best growth situation was pearlite/soil (3:1). The number of bulb scales surviving and regenerating decreased significantly as the increasing of days after the treatment of high temperature 37oC. The regeneration rate can be about 40% for 20days. The effectiveness of eliminating LSV with the method got to more than 95% which was significantly higher compared with the control. |
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