| 中国家鸭的分子遗传多样性及其起源探讨 |
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| 引用本文： | 李慧芳,陈宽维,朱文奇,薛素美,宋卫涛,王光瑛.中国家鸭的分子遗传多样性及其起源探讨[J].农业生物技术学报,2009,17(5):802-807. |
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| 作者姓名： | 李慧芳 陈宽维 朱文奇 薛素美 宋卫涛 王光瑛 |
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| 作者单位： | 1. 江苏省家禽科学研究所,江苏省家禽遗传育种实验室,扬州,225003
2. 福建农林大学动物科学学院,福州,350002 |
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| 基金项目： | 国家自然科学基金项目，国家科技支撑计划项日，江苏省科技服务平台 |
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| 摘 要： | 本研究测定了我国重点保护的8个家鸭品种及斑嘴野鸭的线粒体DNA D-loop区667bp序列，分析了这8种家鸭的遗传多态性和起源。遗传多态性分析结果显示A、G、C、T碱基平均含量分别为25.5%，15.3%，33.4%，25.8%；变异类型为转换和颠换，未发现缺失与插入。确定了20种单倍型，Hap2（A7）为家鸭的主体单倍型型，平均单倍型多样度为0.67136，平均核苷酸多样度为0.00192，攸县麻鸭单倍型多样度、核苷酸多样度最高，8个品种之间双参数遗传距离为0.00056-0.00414，其中连城白鸭和攸县麻鸭之间遗传距离最大。38种单倍型系统发生研究表明，8种家鸭都单一地起源于绿头鸭。
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| 关 键 词： | 家鸭 D-loop区 遗传多样性 起源 |
| 收稿时间： | 2000-8-8 |
| 修稿时间： | 2008-10-16 |
Molecular Genetic Polymorphism and Origin of Chinese Domestic Ducks |
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| Abstract: | The 667 bp control region of mitochondrial DNA from 8 domestic duck breeds and Anas zonorhynchto were sequenced. The genetic polymorphism and the origin of the 8 domestic ducks were analyzed. The result showed that content of nucleotide A, G, C, T were25.5%,15.3%,33.4%and 25.8% respectively. The mutation types were transition and transversion in this region. 20 haplotypes were found in this study, in which Hap2(A7) was major haplotype in 72 domestic ducks. The haplotype diversity(Hd) and average nucleotide diversity(Pi) were 0.67136 and 0.19％, respectively. Hd and Pi of Youxian Sheldrake was the highest in the 8 domestic duck breeds. Kimura 2 parameter genetic distance between the breeds was 0.00056-0.00414. A phylogenetic analysis of the 38
haplotypes identified that the 8 domestic ducks were only origin from Anas platyrhynchos. |
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