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High potential of agro-industrial by-products of pomegranate (Punica granatum L.) as the powerful antifungal and antioxidant substances
Authors:Ali Tehranifar  Yahya SelahvarziMahdiyeh Kharrazi  Vahid Jahan Bakhsh
Institution:a Center of Pomegranate Research, Department of Horticultural Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
b Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract:Microorganisms such as fungi are one of the most important factors that cause oxidative processes during postharvest stage and consequently deterioration of agriculture products would not be unexpected. On the other hand, high antioxidant properties of industrial by-products of pomegranate propose them as powerful antioxidant and antifungal substances. So to investigate the antioxidant and antifungal properties of pomegranate, two independent factorial experiments based on randomized design with 5 replications were conducted. In the first experiment the effect of 3 different parts of pomegranate (peel, seed and leaf) and 2 different kinds of extracts (aqueous and methanolic) with 4 concentrations (0, 500, 1000 and 1500 ppm) were investigated on 3 postharvest fungi (Penicillium italicum, Rhizopus stolonifer and Botrytis cinerea). In the second experiment antioxidant capacity and phenolic content were measured for two different extracts from different parts. Based on the results the methanolic extract showed the highest inhibitory effects on the mycelia growth (IMG) and spore germination (ISG) with 49.82 and 41.25% respectively. On the other hand, peel and seed extracts had more inhibitory effect (IMG and ISG) than leaf extract. The phenolic content of peel extract were also measured 2.8 fold higher than pomegranate leaf extract and antioxidant capacity of peel, seed and leaf extracts of pomegranate were 55.3%, 35.7% and 16.4% respectively. Therefore, it seems that the high percentage of phenolic content in the peel and seed of pomegranate could cause the high antifungal and antioxidant activity of their extracts.
Keywords:Pomegranate  Methanolic extract  Antifungal activity  Phenolic content  Antioxidant activity
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