PCR technique for identification of mussel species |
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| Authors: | Rego Ignacio Martínez Andrés González-Tizón Ana Vieites Juan Leira Francisco Méndez Josefina |
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| Institution: | Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidade da Coru?a, A Zapateira s/n, 15071 La Coru?a, Spain. |
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| Abstract: | Random amplified polymorphic DNA (RAPD) analysis has been applied to the identification of four mussels species: Mytilus edulis, Mytilus chilensis, Mytilus galloprovincialis, and Perna canaliculus. Amplifications of DNA from mussel were carried out using random primers. The most distinctive bands were then isolated, cloned, and sequenced to design specific primers. Finally, DNA from different mussels was amplified with these specific primers, and results allow genetic identification of M. galloprovincialis from the rest of the mussel species. |
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