| 蓝桉果实抗稻瘟菌成分的分离及其活性 |
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| 引用本文： | 谈满良,周立刚,汪冶,郝小江,张仲凯.蓝桉果实抗稻瘟菌成分的分离及其活性[J].植物病理学报,2007,37(5):535-540. |
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| 作者姓名： | 谈满良 周立刚 汪冶 郝小江 张仲凯 |
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| 作者单位： | 1 中国农业大学农学与生物技术学院植物病理学系 北京 100094;2 中国科学院天然产物化学重点实验室 贵阳 550002;3 云南省农业科学院生物技术与种质资源研究所 昆明 620223 |
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| 基金项目： | Hi-Tech Research and Development Program of China (2006AA10A209)，Specialized Research Fund for the Modernization of Traditional Chinese Medicine of Guizhou Province of China (200307)，Foundation of the Key Laboratory for Agro-Bio-technology of Yunnan Province of China (2001B-1) |
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| 摘 要： | 通过抗菌活性测定, 蓝桉(Eucalyptus globulus Labill.)果实乙醇提取物中抗稻瘟菌活性成分主要存在于石油醚萃取部分和乙酸乙酯萃取部分。从石油醚部分和乙酸乙酯部分分离到的15个化合物中, β-桉叶油醇和β-谷甾醇对稻瘟菌孢子萌发具有较强的抑制活性, 它们对稻瘟菌孢子萌发的半抑制浓度(IC50)分别为62.9和141.4μg/mL。其它化合物对稻瘟菌孢子萌发只有轻微的抑制活性。
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| 关 键 词： | 蓝桉 桃金娘科 β-桉叶油醇 β-谷甾醇 稻瘟菌 抗真菌活性 |
| 文章编号： | 0412-0914（2007）05-0535-06 |
| 修稿时间： | 2006-10-08 |
Bioassay-guided Isolation and Inhibitory Activity against Magnaporthe grisea of the Compounds from Fruits of Eucalyptus globulus Labill. |
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| TAN Man-liang, ZHOU Li-gang, WANG Ye, HAO Xiao-jiang, ZHANG Zhong-kai.Bioassay-guided Isolation and Inhibitory Activity against Magnaporthe grisea of the Compounds from Fruits of Eucalyptus globulus Labill.[J].Acta Phytopathologica Sinica,2007,37(5):535-540. |
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| Authors: | TAN Man-liang ZHOU Li-gang WANG Ye HAO Xiao-jiang ZHANG Zhong-kai |
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| Institution: | 1 Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China;2 The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China;3 Institute of Biotechnology and Germplasm Resource, Yunnan Academy of Agricultural Sciences, Kunming 650223, China |
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| Abstract: | It was found that inhibitory compounds against Magnaporthe grisea mainly existed in the petroleum ether fraction and ethyl acetate fraction of the ethanol crude extract of Eucalyptus globulus Labill. fruits. Of fifteen isolated compounds from the both petroleum ether fraction and ethyl acetate fraction, two compounds β-eudesmol and β-sitosterol were screened to show their stronger inhibitory activity on the spore germination than others. The median effective inhibitory concentration (IC50) of β-eudesmol and β-sitosterol against the spore germination of M. grisea was 62.9 μg/mL and 141.4 μg/mL separately. The other compounds were found to have minor inhibitory activity. |
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| Keywords: | Eucalyptus globulus Labill Myrtaceae Magnaporthe grisea antifungal activity |
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